Product Name:
SNCA-pY39
Product Number:
ab-pn850
Target Full Name: Alpha-synuclein
Target Alias: NACP; Non A4 component of amyloid precursor; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; PARK1; PD1; SYN; Synuclein, alpha; SYUA
Product Type Specific: SNCA phosphosite-specific antibody
Antibody Code: PN850
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y39
Protein UniProt: P37840
Protein SigNET: SNCA
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human SNCA
Antibody Immunogen Sequence: EGVL(pY)VGSC
Antibody Immunogen Description: Corresponds to amino acid residues E35 to S42. T39 phosphorylation induces interaction with DRBP1 and inhibits binding to SNCA. This is one of the major phosphorylation sites in SNCA by mass spectrometry (≥15 reports recorded in PhosphoSitePlus). SNCA is known to be phosphorylated at this site in vitro by Abl1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Chicken | Frog.
Related Product 1: SNCA S129 phosphorylation antibody (Cat. No.: AB-PN849)
Scientific Background: SNCA (Alpha-synuclein) is a regulatory protein primarily located in neuronal presynaptic terminals, where it regulates vesicle trafficking and neurotransmitter release. It is a natively unfolded alpha-helical soluble protein that binds lipids but can form pathologic, insoluble amyloid fibril aggregates, contained in Lewy bodies, which are central to Parkinson's disease and other neurodegenerative synucleinopathies. SNCA acts as a dynamic regulator of synaptic function, including trafficking, recycling of synaptic vesicles, and chaperone-mediated functions. It features three main regions: an N-terminus (lipid-binding), a hydrophobic central NAC region (facilitates aggregation), and a highly acidic C-terminus. It participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, it acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. It also functions as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. It lalso plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity. It is highly expressed in the brain, particularly in synaptic terminals, but also found in heart, muscles, and other tissues. Misfolding, post-translational modifications (like phosphorylation at Ser-129), and mutations (e.g., A53T) cause the protein to form toxic oligomers and fibrils. Mutations or overexpression of SNCA are linked to Parkinson’s Disease (PARK1/PARK4), Dementia with Lewy Bodies (DLB), and Multiple System Atrophy (MSA). This description may include information annotated by UniProt and/or Google AI.