Product Name:
PSD95-pS295
Product Number:
ab-pn846
Target Full Name: Disks large homologue 4
Target Alias: PSD 95; PSD-95; DLG4; SAP90; Synapse-associated protein 90; Postsynaptic density protein 95
Product Type Specific: DLG4 (PSD95) synaptic phosphosite-specific antibody
Antibody Code: PN846
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S295
Protein UniProt: P78352
Protein SigNET: PSD95
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PSD95
Antibody Immunogen Sequence: RRY(pS)PVAC
Antibody Immunogen Description: Corresponds to amino acid residues R292 to A298. Phosphorylation of S295 affects neuroplasticity. This is a minor in vivo phosphorylation site in PSD95 (DLG4) (≥12 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus.
Related Product 1: PSD95 expression antibody (Cat. No.: AB-NN142)
Related Product 2: PSD95 expression antibody (Cat. No.: AB-NN142-2)
Related Product 3: PSD95 S73 phosphorylation antibody (Cat. No.: AB-PN900)
Related Product 4: PSD95 Y240 phosphorylation antibody (Cat. No.: AB-PN901)
Scientific Background: Postsynaptic density protein 95 (PSD95) (also known as synapse associated protein 90kDa and DLG4) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex (1). These family members consist of an N-terminal variable segment followed by three amino-terminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins (2). Different PDZ domains of PSD-95 may be specialized for distinct functions (3, 4). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (5). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning (6). Mutations in PSD95 are associated with autism (7). This description may include information annotated by UniProt and/or Google AI.