Product Name:
NR2B-pY1474
Product Number:
ab-pn843
Target Full Name: Glutamate [NMDA] receptor subunit epsilon-2
Target Alias: Glutamate [NMDA] receptor subunit epsilon 2 precursor; Glutamate receptor, ionotropic, N-methyl D-aspartate 2B; HNR3; NMDAR2B; NMDE2; NME2; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NR2B; NR3
Product Type Specific: NR2B (GRN2B) phosphosite-specific antibody
Antibody Code: PN843
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1474
Protein UniProt: Q13224
Protein SigNET: NR2B
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human NR2B
Antibody Immunogen Sequence: GHV(pY)EKLSC
Antibody Immunogen Description: Corresponds to amino acid residues G1471 to S1477. Y1474 has been linked to increased cell motility and regulates synaptic plasticity. This is the major site of phosphorylation of NR2B by mass spectrometry (>105 reports recorded in PhosphoSitePlus). NR2B is known to be phosphorylated at this site in vitro by Fyn, and Src.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.7 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Related Product 1: NR1 S897 phosphorylation antibody (Cat. No.: AB-PN841)
Related Product 2: NR2B S281 phosphorylation antibody (Cat. No.: AB-PN1027)
Scientific Background: NR2B (Glutamate [NMDA] receptor subunit epsilon-2; GRIN2B) is a component of NMDA receptor complexes and belongs to the glutamate-gated ion channel (TC 1.A.10.1) family, NR2B/GRIN2B subfamily. It functions in heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition of the NMDA receptor. The NR2B subunit is specifically responsible for determining the functional properties of the channel, such as high permeability to calcium Ca2+, long-lasting current decay, and sensitivity to pharmacological agents. The surface expression of NR2B is regulated by phosphorylation, for example, at Tyr-1472, which is critical for fear learning and amygdaloid synaptic plasticity. In concert with DAPK1 at extrasynaptic sites, it acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death. NR2Bcontributes to neural pattern formation in the developing brain. NR2B is heavily expressed in the neonatal brain and is gradually replaced by the NR2A subunit in many brain regions (a "subunit switch") as the brain matures. However, in the prefrontal cortex (PFC), expression of NR2B remains high into adulthood. NR2B-containing NMDA receptors are essential for Long-Term Potentiation (LTP), a cellular mechanism for learning and memory. Increased expression of NR2B in the forebrain has been shown to improve learning and memory in mice. It also plays a role in long-term depression (LTD) of hippocampus membrane currents and in synaptic plasticity. It is primarily expressed in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, with lower expression in the basal ganglia. Mutations in the GRIN2B gene cause GRIN2B-related neurodevelopmental disorder, characterized by intellectual disability, delayed speech and motor development, seizures, and sometimes autism spectrum disorder. This description may include information annotated by UniProt and/or Google AI.