Product Name:
NRF2-pS40
Product Number:
ab-pn842
Target Full Name: Nuclear factor erythroid 2-related factor 2
Target Alias: NFE2L2; HEBP1; NRF2
Product Type Specific: NRF2 (NFE2L2) transcription protein phosphosite-specific antibody
Antibody Code: PN842
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S40
Protein UniProt: Q16236
Protein SigNET: NRF2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human NRF2
Antibody Immunogen Sequence: FDF(pS)QRR(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues F37 to R43. S40 phosphorylation induces activity and transcription. In vivo phosphorylation of NRF2 by high throughput mass spectrometry has not been recorded in PhosphoSitePlus. NRF2 is known to be phosphorylated at this site in vitro by PKCa (PRKCA).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Chicken | Zebra fish.
Scientific Background: NF2 (Merlin (moesin-ezrin-radixin-like protein); Schwannomin) is a critical tumour suppressor protein. It is a transcription factor which plays a role in the oxidative stress response. It primarily functions in the nervous system, particularly in Schwann cells, to regulate cell proliferation, shape, and adhesion by acting as a link between the cell membrane and the cytoskeleton. Under normal cellular conditions, it is sequestered in the cytoplasm by Keap1 and Cullin3. When under oxidative stress, Nrf2 is release from Keap1 by p62 and is translocated to the nucleus where it acts as a transcription factor for the expression of oxidative stress-related products. NF2 prevents uncontrolled cell growth and division. Inactivation or loss of this protein leads to neurofibromatosis type 2, characterized by benign tumors like schwannomas, meningiomas, and ependymomas. This description may include information annotated by UniProt and/or Google AI.