Product Name:
NR1-pS897
Product Number:
ab-pn841
Target Full Name: Glutamate [NMDA] receptor subunit zeta-1
Target Alias: N-methyl-D-aspartate receptor subunit NR1
Product Type Specific: NR1 phosphosite-specific antibody
Antibody Code: PN841
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S897
Protein UniProt: Q05586
Protein SigNET: NR1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human NR1
Antibody Immunogen Sequence: RRS(pS)KDTSC
Antibody Immunogen Description: Corresponds to amino acid residues R894 to S900. The effect of NMDAR1 Ser-897 phosphorylation is unclear. In vivo phosphorylation of NR1 by high throughput mass spectrometry has not been recorded in PhosphoSitePlus. NR1 is known to be phosphorylated at this site in vitro by PKACa (PRKACA), and PKCa (PRKCA).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse | Chicken.
Related Product 1: NR2B S281 phosphorylation antibody (Cat. No.: AB-PN1027)
Related Product 2: NR2B Y1474 phosphorylation antibody (Cat. No.: AB-PN843)
Scientific Background: NR1 (Glutamate [NMDA] receptor subunit zeta-1; GluN1; NMDAR1) is an obligatory, essential subunit of the N-methyl-D-aspartate (NMDA) receptors, and belongs to the glutamate-gated ion channel (TC 1.A.10.1) family, NR1/GRIN1 subfamily. NMDA receptors function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. NR1 contains the binding site for glycine, which is a necessary co-agonist. with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition. It functions in excitatory neurotransmission, synaptic plasticity, learning, and memory within the central nervous system (CNS). NR1-containing receptors allow calcium influx, crucial for synaptic plasticity mechanisms like LTP and LTD. NR1 can move to the cell nucleus and may be involved in signaling, especially during pain or excitotoxicity. It features four hydrophobic domains and an intracellular C-terminal domain with sites for phosphorylation and protein interaction. NR1 is widely expressed in the nervous system, with high levels in the hippocampus, cerebral cortex, and cerebellum. Dysfunctional NR1 is linked to various neurological and psychiatric conditions. This description may include information annotated by UniProt and/or Google AI.