Product Name:
Nav1.2-pS568
Product Number:
ab-pn837
Target Full Name: Sodium channel protein type 2 subunit alpha
Target Alias: CIN2; HBSC II; HBSCI; HBSCII; NAC2; Nav1.2; SCN2A1; SCN2A2; Sodium channel protein brain II subunit alpha; Sodium channel protein type II subunit alpha; Sodium channel protein, brain II alpha subunit; Sodium channel type II alpha subunit; Sodium channel, voltage-gated, type II, alpha subunit; Voltage-gated sodium channel subunit alpha Nav1.2
Product Type Specific: Nav1 phosphosite-specific antibody
Antibody Code: PN837
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S568
Protein UniProt: Q99250
Protein SigNET: Nav1.2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human Nav1.2
Antibody Immunogen Sequence: CSLF(pS)PRR
Antibody Immunogen Description: Corresponds to amino acid residues S565 to R571. The effect of S568 phosphorylation is unknown. This is the one of the prevalent phosphosite in Nav1.2 (SCN2A) by mass spectrometry (≥6 reports recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken.
Related Product 1: Nav beta 2 pan-specific antibody (Cat. No.: AB-NN314-1)
Related Product 2: Nav beta 3 pan-specific antibody (Cat. No.: AB-NN315-1)
Scientific Background: Nav1.2 (Sodium channel protein type 2 subunit alpha; SCN2A) is a sodium-selective channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. It belongs to the sodium channel (TC 1.A.1.10) family, Nav1.2/SCN2A subfamily. It is a large, 260 kDa alpha subunit with four repeat domains, each composed of six membrane-spanning segments, often associated with regulatory beta subunits. It takes open or closed conformations in response to the voltage difference across the membrane. The protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. It is suspected to regulate hippocampal replay occurring within sharp wave ripples (SPW-R) important for memory. It is predominantly found on axons and the Axon Initial Segment (AIS) in the brain (cerebral cortex, cerebellum, hippocampus). It is highly expressed in unmyelinated or premyelinated axons and dendrites. Nav1.2 is highly sensitive to blockade by tetrodotoxin (TTX). Pathologic mutations in the SCN2A gene, which encodes the Nav1.2 protein, cause a broad spectrum of neurological conditions. These include early-onset epilepsy (including infantile spasms and Ohtahara syndrome) from gain-of-function mutations. Loss-of-function muations are associated with autism spectrum disorder (ASD), intellectual disability (ID) and development delay. Nav1.2 is the dominant sodium channel in the brain early in life, while NaV1.6 takes over later, which accounts for why SCN2A disorders are often age-dependent. Nav1.2 is tetrodotoxin-sensitive (TTX-sensitive), meaning its activity is blocked by nanomolar concentrations of tetrodotoxin. This description may include information annotated by UniProt and/or Google AI.