Product Name:
MAP2-pS1782
Product Number:
ab-pn835
Target Full Name: Microtubule-associated protein 2
Target Alias: MAP 2; MAP-2; MAP2A; MAP2B; MAP2C; MTAP2
Product Type Specific: MAP2 phosphosite-specific antibody
Antibody Code: PN835
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S1782
Protein UniProt: P11137
Protein SigNET: MAP2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human MAP2
Antibody Immunogen Sequence: IITQ(pS)PGRSC
Antibody Immunogen Description: Corresponds to amino acid residues I1778 to S1786. The effect of MAP2 Ser-1782 phosphorylation is unclear. This is the major in vivo phosphorylation sitesin MAP2 (≥76 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). MAP2 is known to be phosphorylated at this site in vitro by PKACa (PRKACA).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.9 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse | Chicken.
Scientific Background: MAP2 (Microtubule-associated protein 2) is a critical neuronal cytoskeletal protein, primarily located in dendrites. It has a proline-rich domain and a tubulin-binding domain. The precise function of MAP2 is unclear, but MAPs stabilize microtubules, formscross-bridges between them, regulate the spacing and assembly of the cytoskeleton, affect dendritic morphology, and facilitate organelle transport. MAP2 acts as a structural scaffold for neuronal development and is a major marker for identifying neurons. It is abundant in neuronal dendrites and cell bodies (perikarya). MAP2 exists in several isoforms generated by alternative splicing. High-molecular weight MAP2 (MAP2a, MAP2b) is primarily in adult neurons, while low-molecular weight forms (MAP2c, MAP2d) are present during development. MAP2 is a phosphoprotein and its activity is heavily regulated by phosphorylation via kinases like PKA and MAPK, affecting its ability to bind to microtubules. It acts as an anchor for Protein Kinase A (PKA) and contributes to the regulation of organelle transport within dendritic processes. As a marker of synaptic plasticity, MAP2 levels can be used to monitor neuroplasticity following brain injury. This description may include information annotated by UniProt and/or Google AI.