Product Name:
Kv4.2-pT602+pT607
Product Number:
ab-pn833
Target Full Name: Potassium voltage-gated channel subfamily D member 2
Target Alias: KIAA1044; Kv4.2; Potassium channel Kv4.2; Potassium voltage-gated channel, Shal-related subfamily, member 2; RK5
Product Type Specific: Kv4 (KCND2) phosphosite-specific antibody
Antibody Code: PN833
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T602+T607
Protein UniProt: Q9NZV8
Protein SigNET: Kv4.2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human Kv4.2
Antibody Immunogen Sequence: SIP(pT)PPVT(pT)PEGC
Antibody Immunogen Description: Corresponds to amino acid residues S599 to G610. The effect of KCND2 Thr-602 phosphorylation is unclear. Thr-607 phosphorylation stimulates the activity of this potassium channel. These are minor in vivo phosphorylation sites in Kv4.2 based on ≥1 and ≥0 high throughput mass spectrometry reports, respectively, recorded in PhosphoSitePlus. Kv4.2 is known to be phosphorylated at this site in vitro by T602 and T607 by ERK1 (MAPK3), ERK2 (MAPK1). T607: ERK1 (MAPK3), ERK2 (MAPK1).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.7 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Zebra fish.
Related Product 1: Kv3.1 pan-specific antibody (Cat. No.: AB-NN282-1)
Related Product 2: Kv3.2 pan-specific antibody (Cat. No.: AB-NN283-1)
Related Product 3: Kv4.2 S963 phosphorylation antibody (Cat. No.: AB-PN832)
Scientific Background: Kv4.2 (Potassium Voltage-Gated Channel Subfamily D Member 2; KCND2) is the pore-forming alpha-subunit that belongs to the potassium channel family, D (Shal) (TC 1.A.1.2) subfamily. Kv4.2/KCND2 sub-subfamily. It mediates transmembrane potassium transport (the major part of the dendritic A-type current I(SA)) in excitable membranes, primarily in brain neurons. This current is activated at membrane potentials that are below the threshold for action potentials. It regulates neuronal excitability, prolongs the latency before the first spike in a series of action potentials, regulates the frequency of repetitive action potential firing, shortens the duration of action potentials and regulates the back-propagation of action potentials from the neuronal cell body to the dendrites. It participates in the regulation of the circadian rhythm of action potential firing in suprachiasmatic nucleus neurons, which regulates the circadian rhythm of locomotor activity. It functions downstream of the metabotropic glutamate receptor GRM5 and plays a role in neuronal excitability and in nociception mediated by activation of GRM5. It forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane. It can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCND2 and KCND3; channel properties depend on the type of pore-forming alpha subunits that are part of the channel. Interaction with specific isoforms of the regulatory subunits KCNIP1, KCNIP2, KCNIP3 or KCNIP4 strongly increases expression at the cell surface and thereby increases channel activity; it modulates the kinetics of channel activation and inactivation, shifts the threshold for channel activation to more negative voltage values, shifts the threshold for inactivation to less negative voltages and accelerates recovery after inactivation. Likewise, interaction with DPP6 or DPP10 promotes expression at the cell membrane and regulates both channel characteristics and activity. Mutations in KCND2 are associated with early myoclonic encephalopathy, developmental delay, and intellectual disability. Kv4.2 is highly expressed throughout the brain, including in amygdala, caudate nucleus, cerebellum, hippocampus, substantia nigra and thalamus. It is also is present in ovary, in corpus luteum and in granulosa and theca cells in the follicle. Its expression is not detectable or very low in heart, kidney, liver, lung, pancreas and skeletal muscle. This description may include information annotated by UniProt and/or Google AI.