Product Name:
Kv4.2-pS963
Product Number:
ab-pn832
Target Full Name: Solute carrier family 12 member 5
Target Alias: Electroneutral potassium-chloride cotransporter 2; Erythroid K-Cl cotransporter 2; KCC2; KIAA1176; Neuronal K-Cl cotransporter; S12A5; Solute carrier family 12 (potassium-chloride transporter) member 5; Solute carrier family 12, 5
Product Type Specific: KCC2 (SLC12A5) phosphosite-specific antibody
Antibody Code: PN832
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S963
Protein UniProt: Q9H2X9
Protein SigNET: Kv4.2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human Kv4.2
Antibody Immunogen Sequence: SRG(pS)IRR(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues S960 to R966. The effect of KCC2 S963 phosphorylation is unclear. This is one of the major in vivo site of phosphorylation of the KCC2 based on ≥7 mass spectrometry reported recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Rat | Mouse | Platypus | Chicken | Frog.
Related Product 1: Kv3.1 pan-specific antibody (Cat. No.: AB-NN282-1)
Related Product 2: Kv3.2 pan-specific antibody (Cat. No.: AB-NN283-1)
Related Product 3: Kv4.2 T602+T607 phosphorylation antibody (Cat. No.: AB-PN833)
Scientific Background: KCC2 (Solute carrier family 12 member 5; SLC12A5) is an electroneutral potassium-chloride cotransport protein that belongs to the SLC12A transporter family.It is a neuron-specific, ~130-145 kDa transmembrane protein essential for maintaining low intracellular chloride levels in the central nervous system. It acts as a cotransporter, enabling fast hyperpolarizing inhibition by GABA and glycine. It is crucial for mature neuronal excitability, dendritic spine formation. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition. It consists of 12 membrane-spanning segments with intracellular N- and C-terminal domains. It is localized specifically in the cell bodies of neurones, and plasma membrane of neuronal soma and dendrites, particularly at the postsynaptic density, where it regulates dendritic spine formation, synaptic strength and dendritic signal integration. Beyond ion transport, KCC2 interacts with the actin cytoskeleton through its C-terminal domain (CTD) and protein 4.1N, playing a crucial role in dendritic spine morphogenesis and maturation. KCC2 activity is heavily regulated by phosphorylation. Key sites include Ser-940 (phosphorylated by PKC), which enhances surface expression, and Thr-906/Thr-1007 (phosphorylated by WNK kinases), which inhibits transporter activity. inhibitory mutations or reduced expression in KCC2 are linked with various neurological conditions, including epilepsy, traumatic brain injury, schizophrenia, and autism. This description may include information annotated by UniProt and/or Google AI.