Product Name:
HIST1H3A-pS10
Product Number:
ab-pn826
Target Full Name: Histone H3.3
Target Alias: CG5825; H3.3; H3.3Q; H3.A; H3.B; H33; H3f3a; H3F3B; HIS3.3A; HIS3.3B ORCG8989; HISH3-3Q
Product Type Specific: H3F3A phosphosite-specific antibody
Antibody Code: PN826
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S10
Protein UniProt: P84243
Protein SigNET: HIST1H3A
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human HIST1H3A
Antibody Immunogen Sequence: TARK(pS)TGGC
Antibody Immunogen Description: Corresponds to amino acid residues T601 to G13. The effect of S10 phosphorylation is unclear. This is a minor in vivo site of phosphorylation of H3F3A (≥4 reports from high throughput mass spectrometry studies in PhosphoSitePlus). H3F3A is known to be phosphorylated at this site in vitro by Akt1 (PKBa), AurKB (AurB, STK12), Fyn, IKKa (CHUK, IKBKA), MSK1 (RPS6KA5), MSK2 (RPS6KA4), PKACa (PRKACA), RSK1 (RPS6KA1), RSK2 (RPS6KA3), and SNRK.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Mouse.
Scientific Background: H3F3A (H3-3A, H3.3 histone A) is a replication-independent variant histone protein H3.3, which is incorporated into chromatin to regulate gene expression, particularly at active gene promoters. It constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. H3F3A (H3.3) acts in conjunction with H3F3B to maintain chromatin structure and genome integrity during mammalian development. Mutations in H3F3A, notably K27M and G34R/V, act as "oncohistones" by causing global epigenetic changes that drive specific pediatric-type high-grade gliomas (i.e., K27M) and giant cell tumours of bone (i.e., G34R/V). The K27M mutation results in global loss of H3K27me3, leading to oncogenic transcription. This description may include information annotated by UniProt and/or Google AI.