Product Name:
H2AX-pS140
Product Number:
ab-pn824
Target Full Name: Histone H2A.X
Target Alias: H2A histone family, member X; H2A.X; H2a/x; HIST5-2AX; Histone H2A.X
Product Type Specific: H2AFX phosphosite-specific antibody
Antibody Code: PN824
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S140
Protein UniProt: P16104
Protein SigNET: H2AX
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human H2AX
Antibody Immunogen Sequence: CATQA(pS)QEY(-cooh)
Antibody Immunogen Description: Corresponds to amino acid residues A136 to Y143. Phosphorylation of S140 regulates molecular associations and intracellular location. This is a significant site of phosphorylation by mass spectrometry (≥58 reports by mass spectrometry in PhosphoSitePlus). H2AFX is known to be phosphorylated at this site in vitro by ATM, DNAPK, MST1, JNK1, and JNK2.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Mouse | Frog.
Scientific Background: H2AFX (H2A histone family, member X) is a histone H2A variant that is crucial for crucial for DNA repair, specifically double-strand breaks (DSBs), and plays a role in V(D)J recombination, chromatin remodeling, and cell cycle checkpoint activation. This variant of histone H2A which replaces conventional H2A in a subset of nucleosomes. It forms a key part of nucleosomes and is activated by phosphorylation at Ser-140 in response to DNA double-strand breaks, recruiting repair factors. It is often overexpressed in various cancers, including neuroblastoma and breast cancer, and its altered expression acts as a prognostic biomarker and is linked to genomic instability. This description may include information annotated by UniProt and/or Google AI.