Product Name:
GluR1-pS849
Product Number:
ab-pn822
Target Full Name: Glutamate receptor 1
Target Alias: AMPA-selective glutamate receptor 1; GLR1; GLUH1; GLUR1; GluR-1; GLURA; GluR-A; GluR-K1; Glutamate receptor ionotropic, AMPA 1; Glutamate receptor, ionotropic, AMPA 1; GRIA1
Product Type Specific: GluR1 phosphosite-specific antibody
Antibody Code: PN822
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S849
Protein UniProt: P42261
Protein SigNET: GluR1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human GluR1
Antibody Immunogen Sequence: C(βA)PQQ(pS)INE
Antibody Immunogen Description: Corresponds to amino acid residues P846 to E852. Y849 phosphorylation induces activity. This is the major site of phosphorylation of GluR1 in vivo with ≥7 mass spectrometry reports in PhosphoSite Plus GluR1 is known to be phosphorylated at this site in vitro by CAMK2A, and PKCA.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.7 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Platypus.
Related Product 1: GluA1/GluR1 Glutamate Receptor expression antibody (Cat. No.: AB-NN263-1)
Related Product 2: GluN1/NR1 expression antibody (Cat. No.: AB-NN264-1)
Related Product 3: GluN2A/NR2A expression antibody (Cat. No.: AB-NN297-1)
Related Product 4: GluN2A/NR2A expression antibody (Cat. No.: AB-NN297-2)
Related Product 5: GluR2 Y876 phosphorylation antibody (Cat. No.: AB-PN823)
Scientific Background: GluR1 (Glutamate receptor 1) is an ionotropic receptor that belongs to the glutamate-gated ion channel (TC 1.A.10.1) family, GRIA1 subfamily. It is a key subunit of the ionotropic α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors (AMPARs) and its ligand L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. L-glutamate and other glutamatergic agonists such as AMPA, quisqualic acid, and kainic acid can act as ligands for this receptor. Binding of these agonists to GluR1 induces a conformation change, leading to the opening of the cation channel, and conversion of the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state with the bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, GLuR1 shows resensitization which is characterized by a delayed accumulation of current flux upon continued exposure to glutamate. As part of tetrameric receptor complexes, GluR1 is essential for synaptic plasticity, memory formation, and long-term potentiation (LTP). Reversible phosphorylation of Ser-831 by CaMK2, and Ser-845 by PKA) on the C-terminus regulates single-channel conductance and synaptic trafficking. GluR1 is widely expressed in brain, particularly in the hippocampus and cortex. It is implicated in neurological disorders, including schizophrenia, depression, and epilepsy, as well as autoimmune encephalitis. This description may include information annotated by UniProt and/or Google AI.