Product Name:
CFL1-pS3
Product Number:
ab-pn812
Target Full Name: Cofilin-1
Target Alias: CFL; COF1; Cofilin; Cofilin 1 (non-muscle); Cofilin, non-muscle isoform; Non-muscle isoform; P18
Product Type Specific: Cofilin phosphosite-specific antibody
Antibody Code: PN812
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S3
Protein UniProt: P23528
Protein SigNET: CFL1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human CFL1
Antibody Immunogen Sequence: MA(pS)GVAC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to A6. S3 phosphorylation has been reported to both inhibit and stimulate activity. This is one of the common in vivo phosphorylation site in cofilin-1 (≥72 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). Cofilin 1 is known to be phosphorylated at this site in vitro by LIMK1, and LIMK2.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish.
Related Product 1: CFL1 Y140 phosphorylation antibody (Cat. No.: AB-PN813)
Scientific Background: CFL1 (Cofilin-1) is a 15-20 kDa cytoskeletal protein in the actin-binding proteins ADF family that binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. it is important for regulating actin cytoskeleton dynamics by severing and depolymerizing ADP-actin filaments. It promotes cell motility, division, and intracellular transport by recycling actin monomers. Activity is regulated via phosphorylation (inactive) and pH. It functions in the regulation of cell morphology and cytoskeletal organization. It is required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. It is also needed for neural tube morphogenesis and neural crest cell migration. Cofilin 1 is inactivated by phosphorylation at serine 3 (Ser3) by LIM kinases (LIMK) and activated by dephosphorylation by phosphatases such as slingshot (SSH). Phosphoinositides (PIP2) and high pH can also inhibit its binding to actin. It is widely expressed in various non-muscle tissues. Over-expression is linked to cancer metastasis, and dysfunction is associated with neurodegenerative diseases.This description may include information annotated by UniProt and/or Google AI.