Product Name:
Bcl-2-pS70
Product Number:
ab-pn811
Target Full Name: Apoptosis regulator Bcl-2
Target Alias: B-cell CLL/lymphoma 2; BCL2
Product Type Specific: Bcl2 phosphosite-specific antibody
Antibody Code: PN811
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S70
Protein UniProt: P10415
Protein SigNET: Bcl2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human Bcl-2
Antibody Immunogen Sequence: CART(pS)PLQT
Antibody Immunogen Description: Corresponds to amino acid residues A67 to T74. S70 phosphorylation of Bcl-2 inhibits its activity. Bcl2 is known to be phosphorylated at this site in vitro by JNK, and JNK3.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Rat | Mouse.
Scientific Background: Bcl2 (B-cell lymphoma 2) is a 26 kDa integral membrane protein that belongs to the Bcl-2 family, and regulate apoptosis (programmed cell death). It functions as a potent anti-apoptotic protein, crucial for promoting cell survival by preventing mitochondrial outer membrane permeabilization (MOMP), thereby inhibiting the release of cytochrome c and subsequent caspase-mediated cell death. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability. It functions in a feedback loop system with caspases. and Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). It may attenuate inflammation by impairing NLRP1-inflammasome activation, and CASP1 activation and IL1B release. It is widely expressed in a variety of tissues, and is primarily located on the outer mitochondrial membrane, endoplasmic reticulum, and nuclear envelope. Overexpression of Bcl-2, often due to translocation t(14;18), which puts it under the control of the IgH enhancer, is frequently observed in follicular lymphomas and various other malignancies. This description may include information annotated by UniProt and/or Google AI.