Product Name:
APP-pT743
Product Number:
ab-pn808
Target Full Name: Amyloid beta A4 protein
Target Alias: A4; ABPP; AD1; Alzheimer disease amyloid protein; Alzheimer's disease amyloid protein; Amyloid beta (A4) precursor protein; APPI; Beta-amyloid protein 40; Beta-amyloid protein 42; C31; C83; Cerebral vascular amyloid peptide; CVAP; Gamma-CTF(50); Gamma-CTF(57); Gamma-CTF(59); P3(40); P3(42); Peptidase nexin-II; PN-II; PreA4; Protease nexin-II; Soluble APP-alpha (S-APP- alpha); Soluble APP-beta (S-APP-beta)
Product Type Specific: APP phosphosite-specific antibody
Antibody Code: PN808
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T743
Protein UniProt: P05067
Protein SigNET: APP
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human APP
Antibody Immunogen Sequence: CAAV(pT)PEE
Antibody Immunogen Description: Corresponds to amino acid residues A740 to E746. Phosphorylation of T743 regulates protein location and interactions. APP is known to be phosphorylated at this site in vitro by CDK1 and LRRK2.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.9 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse | Chicken | Frog | Zebra fish | Tiger blowfish | Nematode worm.
Related Product 1: APP expression antibody (Cat. No.: AB-NN192-3)
Related Product 2: APP Y757 phosphorylation antibody (Cat. No.: AB-PN809)
Scientific Background: Amyloid monomeric proteins can sometimes oligomerize into destructive amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation. Many degenerative diseases are known to be related to the accumulation of misfolded proteins as amyloid fibres (1, 2). These include the amyloid-β peptide plaques and tau neurofibrillary tangles in senile plaques of Alzheimer’s symptomology, the deposition of α-synuclein in the Lewy bodies of Parkinson’s disease, and accumulation of polyglutamine-containing aggregates in Huntington’s disease (2, 3). Amyloid Precursor Protein (APP) is a type-I transmembrane glycoprotein, primarily expressed in the brain, that plays a critical role in synaptic formation, repair, and neuronal signaling. It is the precursor to the amyloid-beta (Ab) peptide, which, when misprocessed, forms pathogenic amyloid plaques in Alzheimer's disease. Abnormal cleavage of APP by beta-secretase and gamma-secretase releases toxic peptides, which aggregate into neurotoxic extracellular plaques. APP features a large extracellular N-terminal domain, a single transmembrane domain, and a short intracellular C-terminal domain (AICD). It is normally highly expressed in the nervous system, and is involved in cell adhesion, copper transport, synaptic plasticity and long-term potentiation. This description may include information annotated by UniProt and/or Google AI.