Product Name:
TRHR-pS364+pT365
Product Number:
ab-pn806
Target Full Name: Thyrotropin-releasing hormone receptor
Target Alias: Thyroliberin receptor; TRFR; TRH-R
Product Type Specific: TRHR phosphosite-specific antibody
Antibody Code: PN806
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S364+T365
Protein UniProt: P34981
Protein SigNET: TRHR
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human TRHR
Antibody Immunogen Sequence: C(βA)DHF(pS)(pT)ELD
Antibody Immunogen Description: Corresponds to amino acid residues D361 to D368. S364 and T365 phosphorylation leads to internalization of the receptor. These have not been identified as in vivo phosphorylation sites of TRHR based on mass spectrometry reports recorded in PhosphoSitePlus. TRHR is known to be phosphorylated in vitro at S364 by BARK1 (GRK2, ADRBK1), and at T365 by BARK1 (GRK2, ADRBK1), and CK2a1 (CSNK2A1).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Chicken | Zebra fish.
Scientific Background: TRHR (Thyrotropin-releasing hormone receptor) is a 7 transmembrane domain G protein-coupled receptor that belongs to the G protein coupled receptor 1 family. It is the receptor for thyrotropin-releasing hormone. This receptor is mediated by G proteins (Gaq and Ga11), which leads to the stimulation of phospholipase C (PLC), resulting in the formation of diacylglycerol (DAG) and inositol triphosphate (IP3), which in turn leads to the release of calcium from calcisomes, and increases cytoplasmic Ca2+. This triggers the release of thyroid-stimulating hormone (TSH), prolactin, and growth hormone. TRHR is found primarily on anterior pituitary thyrotropes, playing a critical role in thyroid hormone homeostasis with control of the hypothalamic-pituitary-thyroid (HPT) axis, which is vital for maintaining metabolism. Beyond thyroid regulation, it acts as a central nervous system regulator, impacting arousal and autonomic functions in the brain, pancreas, and reproductive organs. Mutations in TRHR are associated with TRH resistance, and it is a target for treating central nervous system disorders. This description may include information annotated by UniProt and/or Google AI.