Product Name:
TBXA2R-pS329+pS331
Product Number:
ab-pn805
Target Full Name: Thromboxane A2 receptor
Target Alias: Prostanoid TP receptor
Product Type Specific: TBXA2R phosphosite-specific antibody
Antibody Code: PN805
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S329+S331
Protein UniProt: P21731
Protein SigNET: TBXA2R
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human TBXA2R
Antibody Immunogen Sequence: RPR(pS)L(pS)LQP(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues R326 to P334. S329 and S331 phosphorylation by PKC-alpha leads to desensitization of the receptor. TBXA2R is known to be phosphorylated in vitro at S329 by PKACa (PRKACA), and PKCa (PRKCA).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog.
Scientific Background: TBXA2R (Thromboxane A2 receptor) is a 7-transmembrane, G protein-coupled receptor for thromboxane (TXA2), and belongs to the rhodopsin-like GPCR family (family 1). It is crucial for inducing platelet aggregation, and vasoconstriction of smooth muscles. There are two isoforms of TBXA2R in humans, TPa (Isoform 1) and TPb (Isoform 2), derived from alternative splicing of the TBXA2R gene. The functional activities of this receptor is mediated by G proteins. In the kidney, particularly in glomeruli, arterioles, and mesangial cells, with the binding of TXA2 to glomerular thromboxane prostanoid (TP receptors) induces potent vasoconstriction, leading to reduced glomerular blood flow and lowered glomerular filtration rate (GFR). Activation of the TP receptor by TXA2 or its precursor PGH2 primarily couples with Gq proteins, activating phospholipase C (PLC) and resulting in increased intracellular Ca2+ and protein kinase C (PKC) activation. It also couples with Gs and Gi proteins to regulate adenylate cyclase and cAMP production: Isoform 1 activates adenylyl cyclase, and Isoform 2 inhibits adenylyl cyclase. TBXA2R is expressed in tissues such as platelets, vascular smooth muscle, and the kidney. It has been implicated in conditions like myocardial infarction, stroke, asthma, and triple-negative breast cancer (TNBC). TBXA2R is essential for the survival and migration of certain TNBC cells. Mutations in the protein are linked to platelet bleeding disorders (e.g., BDPLT13). It is also involved in cerebrovascular disease and aspirin-intolerant asthma. This description may include information annotated by UniProt and/or Google AI.