Product Name:
SSTR2-pS341+pS343
Product Number:
ab-pn804
Target Full Name: Somatostatin receptor type 2
Target Alias: Somatostatin receptor 2; SRIF-1; SS2R; SSR2
Product Type Specific: SSTR2 phosphosite-specific antibody
Antibody Code: PN804
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S341+S343
Protein UniProt: P30874
Protein SigNET: SSTR2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human SSTR2
Antibody Immunogen Sequence: CGER(pS)D(pS)KQD
Antibody Immunogen Description: Corresponds to amino acid residues G338 to D346. S341 and S343 phosphorylation leads to desensitization and internalization of the receptor. These have not been reported as in vivo phosphorylation sites in SSTR2 based high throughput mass spectrometry reports in PhosphoSitePlus. SSTR2 is known to be phosphorylated in vitro at S341 by BARK1 (GRK2, ADRBK1), BARK2 (GRK3, ADRBK2), and at S343 by BARK1 (GRK2, ADRBK1), BARK2 (GRK3, ADRBK2), and PKCa (PRKCA).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish.
Scientific Background: SSTR2 (Somatostatin receptor 2) is a 7-transmembrane G protein-coupled receptor that acts as the primary, highly expressed target for somatostatin-14 and -28 in neuroendocrine cells. It inhibits hormone release (GH, insulin) and cell proliferation. It is a critical diagnostic and therapeutic target for neuroendocrine tumours (NETs). There are two main isoforms (A and B). It inhibits adenylyl cyclase and reduces calcium-dependent exocytosis, leading to decreased hormone secretion. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. It inhibits calcium entry by suppressing voltage-dependent calcium channels. It inhibits cell growth through enhancement of MAPK1 and MAPK2 phosphorylation and subsequent up-regulation of CDKN1B. It stimulates neuronal migration and axon outgrowth and may participate in neuron development and maturation during brain development. It mediates negative regulation of insulin receptor signaling through PTPN6. It inactivates SSTR3 receptor function following heterodimerization. It belongs to the G protein coupled receptor 1 family. It is expressed in both pancreatic alpha- and beta-cells, the cerebrum and kidney and, in lesser amounts, in the jejunum, colon and liver. In the developing nervous system, it is expressed in the cortex where it is located in the preplate at early stages and is enriched in the outer part of the germinal zone at later stages. In the cerebellum, expressed in the deep part of the external granular layer at gestational week 19. This pattern persists until birth but disappears at adulthood. It promotes apoptosis (cell death) and inhibits cell proliferation in cancer cells. It is highly expressed in the cerebrum, kidney, and pancreatic islets. It promotes apoptosis (cell death) and inhibits cell proliferation in cancer cells. This description may include information annotated by UniProt and/or Google AI.