Product Name:
P2RY1-pS352+pS354
Product Number:
ab-pn803
Target Full Name: P2Y purinoceptor 1
Target Alias: ATP receptor; P2 purinoceptor subtype Y1; P2Y1; Platelet ADP receptor; Purinergic receptor
Product Type Specific: P2RY1 phosphosite-specific antibody
Antibody Code: PN803
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S352+S354
Protein UniProt: P47900
Protein SigNET: P2RY1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human P2RY1
Antibody Immunogen Sequence: CANLQ(pS)K(pS)ED
Antibody Immunogen Description: Corresponds to amino acid residues A348 to D356. S352 and S354 phosphorylation leads to desensitization of the receptor by promoting its internalization. These are two of the major in vivo phosphorylation sites in P2RY1 by mass spectrometry (≥2 and ≥2 reports, respectively, in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse | Platypus | Chicken |.
Scientific Background: P2RY1 (P2Y purinoceptor 1) is a 7-transmembrane G protein-coupled receptor (7TM GPCR) of the Type 1 G protein-coupled receptor family. It functions as a major receptor for extracellular adenosine diphosphate (ADP) and adenosine triphosphate (ATP). The receptor activates Gq class G proteins, leading to the stimulation of phospholipase C (PLC) and the mobilization of intracellular calcium ions (Ca2+). It also regulates the PI3K/Akt/GSK3 axis depending in astrocytes and neural cells, and in platelets. Alongside P2RY12, P2RY1 is crucial for ADP-induced platelet shape change and aggregation. It also mediates cellular processes such as calcium signalling, proliferation, endothelial cell migration, alveolar bone resorption, purinergic signaling in the nervous system, thrombosis and apoptosis. In astrocytes, P2RY1 signalling inhibits Glycogen Synthase Kinase-3 beta (GSK3b) by promoting its phosphorylation at its inhibitory Ser- 9 site in a PKC-dependent manner that does not require Akt. P2RY1 is expressed in platelets, megakaryoblastic cells, skeletal muscle fibers, and various neural tissues, and located in the plasma membrane. P2RY1 is linked to bleeding disorders and thrombotic disorders. This description may include information annotated by UniProt and/or Google AI.