Product Name:
LTB4R-pT308+pS310
Product Number:
ab-pn800
Target Full Name: Leukotriene B4 receptor 1
Target Alias: BLT1; BLTR; Chemoattractant receptor-like 1; CMKRL1; GPR16; G-protein coupled receptor 16; Leukotriene B4 receptor; LT4R1; LTB4R1; LTB4-R1; P2RY7; P2Y purinoceptor 7; P2Y7
Product Type Specific: LTB4R phosphosite-specific antibody
Antibody Code: PN800
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T308+S310
Protein UniProt: Q15722
Protein SigNET: LTB4R
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human LTB4R
Antibody Immunogen Sequence: EG(pT)G(pS)EAC
Antibody Immunogen Description: Corresponds to amino acid residues E306 to A312. T308 and S310 phosphorylation is inhibitory to downstream signalling by inducing receptor desensitization. These are minor in vivo phosphorylation sites in LTB4R (≥2 and ≥2 reports, respectively, from high throughput mass spectrometry studies recorded in PhosphoSitePlus). LTB4R is known to be phosphorylated in vitro at T308 by GRK6 (GPRK6).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Scientific Background: LTB4R (Leukotriene B4 Receptor 1; BLT1) is a 7-transmembrane G protein-coupled receptor (7TM GPCR) of the G protein-coupled receptor 1 family. It is a high affinity receptor for Leukotriene B4, and extracellular ATP > UTP and ADP. It is primarily expressed on leukocytes, and also acts as a high-affinity receptor for the potent chemoattractant leukotriene LTB4. The activity of this receptor is mediated by pertusissis toxin-senstive Gi-like G proteins which activate a phosphatidylinositol C-calcium second messenger system. Via activation of MAPK's and NF-kappa B, it triggers inflammatory responses, including chemotactic migration of neutrophils, macrophages, and leukocytes to sites of tissue injury and infection, leukocyte activation, and cytokine production. It may also be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. It is Implicated in inflammatory diseases, including asthma, arthritis and atherosclerosis, psoriasis, and cancer progression (e.g., renal cell carcinoma). LTB4R is predominantly found on peripheral leukocytes (neutrophils, monocytes, T-cells, mast cells). It is highly expressed in spleen, thymus, heart, skeletal muscle and at lower levels in brain and liver. This description may include information annotated by UniProt and/or Google AI.