Product Name:
KMT2C-pS4267
Product Number:
ab-pn798
Target Full Name: Histone-lysine N-methyltransferase MLL3
Target Alias: HALR; Histone-lysine N-methyltransferase, H3 lysine-4 specific MLL3; KIAA1506; KMT2C; Myeloid/lymphoid or mixed-lineage leukemia 3; Myeloid/lymphoid or mixed-lineage leukemia protein 3 homologue
Product Type Specific: KMT2C (MLL3) phosphosite-specific antibody
Antibody Code: PN798
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S4267
Protein UniProt: Q8NEZ4
Protein SigNET: KMT2C
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human KMT2C
Antibody Immunogen Sequence: LPQ(pS)PMR(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues L4264 to R4270. The effect of S4267 phosphorylation is unclear. This is the major in vivo phosphorylation site in KMT2C based on ≥52 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.6 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Rat | Mouse.
Scientific Background: KMT2C (Lysine Methyltransferase 2C; MLL3) is a nuclear histone methyltransferase that belongs in the histone-lysine methyltransferase family, TRX/MLL subfamily. It adds methyl groups to the Lys-4 position of histone H3 (H3K4me1/2), which activates enhancers and facilitates gene transcription. It catalyzes the reaction: L-lysyl4-[histone H3] + S-adenosyl-L-methionine = N6-methyl-L-lysyl4-[histone H3] + S-adenosyl-L-homocysteine + H+. It regulates gene expression, differentiation, and DNA repair within the COMPASS-like complex. It works closely with its paralog, KMT2D (MLL4). It features a SET domain (catalytic activity), PHD-type zinc fingers, and a RING-type zinc finger. It acts as a tumour suppressor by mediating histone H3 lysine 4 (H3K4) mono- and di-methylation at enhancer regions. Loss-of-function mutations in KMT2C are frequently found in various cancers (e.g., gastric, prostate, leukemia). It is also associated with neurodevelopmental disorders such Kleefstra syndrome-2. It is highly expressed in testis and ovary, followed by brain and liver. It is also expressed in placenta, peripherical blood, fetal thymus, heart, lung and kidney. Within brain, expression was highest in hippocampus, caudate nucleus, and substantia nigra. It does not appear to be detectable in skeletal muscle and fetal liver. This description may include information annotated by UniProt and/or Google AI.