Product Name:
GABBR1-pT873
Product Number:
ab-pn796
Target Full Name: Gamma-aminobutyric acid type B receptor subunit 1 precursor
Target Alias: GABA-B receptor; GABA-B receptor 1; GABAB(1e); GABAB, subunit 1c; GABABR1; GABA-B-R1; GABBR1-3; GABR1; Gamma-aminobutyric acid (GABA) B receptor, 1; Gamma-aminobutyric acid type B receptor, subunit 1; Gb1; GPRC3A; HGB1a
Product Type Specific: GABBR1 G protein-coupled receptor phosphosite-specific antibody
Antibody Code: PN796
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T873
Protein UniProt: Q9UBS5
Protein SigNET: GABBR1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human GABBR1
Antibody Immunogen Sequence: AQD(pT)MKTGC
Antibody Immunogen Description: Corresponds to amino acid residues A870 to T876. T873 phosphorylation is likely to be inhibitory to downstream signalling. This is one of the major in vivo phosphorylation sites with ≥6 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.35 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse.
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Scientific Background: GABA (γ-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system, and it reduces the ability of nerve cells to send or receive chemical messages, producing a calming effect to counteract anxiety, stress and fear. This small molecule interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptors. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kDa protein and GABA(B)R1b is a 95 kDa protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1). This description may include information annotated by UniProt and/or Google AI.