Product Name:
DOR1-pT358+pT361+pS363
Product Number:
ab-pn795
Target Full Name: Delta-type opioid receptor
Product Type Specific: DOR1 phosphosite-specific antibody
Antibody Code: PN795
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T358+T361+S363
Protein UniProt: P41143
Protein SigNET: DOR1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human DOR1
Antibody Immunogen Sequence: ERV(pT)AS(pT)P(pS)DGPGC
Antibody Immunogen Description: Corresponds to amino acid residues E355 to G367. T358, T361 and S363 phosphorylation of the S363 site by GRK2 leads to desensitization of the receptor to its agonist and internalization of the receptor. These are minor in vivo phosphorylation sites in DOR1 (≥0, ≥1 and ≥1 reports, respectively, from high throughput mass spectrometry studies recorded in PhosphoSitePlus). DOR1 is known to be phosphorylated in vitro at T361 by GRK2, GRK3. S363: GRK2, and GRK3.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.8 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse | Chicken.
Scientific Background: DOR1 (Delta-type opioid receptor 1; OPRD1) is a 30.5 kDa 7-transmembrane G protein-coupled receptor (7TM GPCR) that belongs to the G protein-coupled receptor 1 family. It functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (Gi/Go proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. DOR1 signalling leads to the inhibition of adenylate cyclase activity. It inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. It functions in the perception of pain and in opiate-mediated analgesia, and plays a role in developing analgesic tolerance to morphine. It is expressed in the plasma membrane and vesicles of neurons, particularly in the brain's basal ganglia and neocortical regions (cerebral cortex), hypothalamus, hippocampus, olfactory bulb and oocytes. Variants in the OPRD1 gene are associated with susceptibility to opioid dependence and heroin use. It has also been linked to skin aging, melanin expression, and gout. Its expression is reduced in the amygdala and putamen in Alzheimer's disease and Parkinson's disease. This description may include information annotated by UniProt and/or Google AI.