Product Name:
DARPP32-pY74
Product Number:
ab-pn794
Target Full Name: Dopamine- and cAMP-regulated neuronal phosphoprotein 32 kDa protein
Target Alias: Dopamine and cAMP regulated phosphoprotein; Dopamine- and cAMP-regulated neuronal phosphoprotein; FLJ20940; IPPD; Neuronal phosphoprotein DARPP-32; PPP1R1B; PPR1B; Protein phosphatase 1, regulatory (inhibitor) subunit 1B
Product Type Specific: DARPP32 phosphosite-specific antibody
Antibody Code: PN794
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y74
Protein UniProt: Q9UD71
Protein SigNET: DARPP32
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human DARPP32
Antibody Immunogen Sequence: C(βA)A(pY)TPPS
Antibody Immunogen Description: Corresponds to amino acid residues A73 to S78. The effect of Y74 phosphorylation is unclear. This is a major site of in vivo phosphorylation with 52 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.8 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rat | Mouse.
Related Product 1: DARPP32 T75 phosphorylation antibody (Cat. No.: AB-PN818)
Scientific Background: DARPP32 (Protein phosphatase 1 regulatory subunit 1B; PPP1R1B) belongs to the protein phosphatase inhibitor 1 family. It mediates dopamine, glutamate, and other neurotransmitter signals in medium-size spiny neurons, regulating responses to antipsychotics and drugs of abuse. It serves as a central hub, integrating signals from various neurotransmitters (dopamine, glutamate, serotonin) and mediating actions of stimulants like cocaine, nicotine, and caffeine. It is a potent inhibitor of protein phosphatase-1 (PP1) when phosphorylated by protein kinase A (PKA) at Thr-34. It has also been implicated as a PKA inhibitor when phosphorylated at Thr-75 by CDK5. DARPP32 activity is further modulated by phosphorylation at other sites (Ser-97, Ser-102, Ser-130) by kinases such as CK1 and CK2, which regulate its subcellular localization. It is highly concentrated in the basal ganglia, specifically in dopaminoceptive neurons (medium spiny neurons). It is also found in other brain regions and certain peripheral tissues (e.g., adrenal cortex). It regulates various physiological processes, including neural plasticity, motor activity, and drug addiction. Altered DARPP-32 expression is linked to neurological and psychiatric conditions, including schizophrenia, bipolar disorder, ADHD (due to its role in dopamine pathways) and Alzheimer's disease (via cleavage by calpain). It is expressed in certain epithelial cells and implicated in cancers such as gastric and colorectal cancer, where it can promote cell invasion. This description may include information annotated by UniProt and/or Google AI.