Product Name:
CHRM1-pS451+pT455+pS457
Product Number:
ab-pn790
Target Full Name: Muscarinic acetylcholine receptor M1
Product Type Specific: CHRM1 phosphosite-specific antibody
Antibody Code: PN790
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S451+T455+S457
Protein UniProt: P11229
Protein SigNET: CHRM1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human CHRM1
Antibody Immunogen Sequence: G(pS)VHR(pT)P(pS)RQC
Antibody Immunogen Description: Corresponds to amino acid residues G450 to Q459. S451, T455 and S457 phosphorylation is likely to be inhibitory to downstream signalling. These are minor in vivo phosphorylation sites in Chk2 (≥4, ≥3 and ≥3 reports, respectively from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse.
Scientific Background: CHRM1 (Muscarinic acetylcholine receptor M1; mAChR m1) is a 51 kDa 7-transmembrane domain receptor that belongs to the G protein-coupled receptor (1 family (7TM GPCR), Muscarinic acetylcholine receptor subfamily, CHRM1 sub-subfamily. It binds the neurotransmitter acetylcholine to modulate neuronal activity, memory consolidation, cognitive processing, and synaptic plasticity. Downstream signalling includes inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Its primary transducing effect is Pi turnover. It is mainly expressed in the brain (cortex, hippocampus), but also involved in vagally induced bronchoconstriction. It is essential for regulating REM sleep, often acting in concert with CHRM3. Reduced expression of CHRM1 is linked to schizophrenia. Its inhibition is associated with deliriant effects (hallucinations/delirium). It interacts with BACE1, a key enzyme in Alzheimer's disease, and may modulate its proteasomal degradation. This description may include information annotated by UniProt and/or Google AI.