Product Name:
BKR2-pS366+pT369
Product Number:
ab-pn788
Target Full Name: B2 bradykinin receptor
Target Alias: BDKRB2; BK-2 receptor
Product Type Specific: BKR2 phosphosite-specific antibody
Antibody Code: PN788
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S366+T369
Protein UniProt: P30411
Protein SigNET: BKR2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human BKR2
Antibody Immunogen Sequence: MEN(pS)MG(pT)LR(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues M363 to R371. S366 and T369 phosphorylation is likely to be inhibitory to downstream signalling. BKR2 is known to be phosphorylated in vitro at S366: by GRK4.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse | Playtpus.
Scientific Background: BKR2 (B2 bradykinin receptor) is a 7-transmembrane G protein-coupled receptor (7TM GPCR) that belongs to the G protein-coupled receptor Type 1 family, Bradykinin receptor subfamily. Bradykinin (released during trauma and inflammation) and kallidin (lys-bradykinin) act as ligands for this receptor. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. Upon ligand binding, BKR2 activates Gq and Gi proteins, resulting in the stimulation of phospholipase C (PLC) and an increase in intracellular free calcium. It also activates the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways. It plays an important role in mediating physiological processes such as vasodilation, inflammation, and pain sensation, and is constitutively expressed (always present) in many tissues. It is ubiquitous, and highly expressed in normal smooth muscle tissue, fibroblasts, and neurons. It plays a critical role in regulating the cardiovascular system, mediating inflammation, and controlling vascular tone. BKR2 activation mediates vasodilation, vascular permeability (edema), smooth muscle spasm, and pain fiber stimulation. It helps lower blood pressure and serves as a functional antagonist to angiotensin II. BKR2 is crucial for tissue protection in the kidneys (preventing diabetic nephropathy) and mediates vascular repair by recruiting angiogenic progenitor cells to sites of injury. Its dysfunction is linked to hereditary angioedema (HAE) and cancer progression (e.g., in gliomas), where it acts as a chemoattractant promoting cell migration.This description may include information annotated by UniProt and/or Google AI.