Product Name:
AVPR1A-pT378+pS380
Product Number:
ab-pn787
Target Full Name: Vasopressin V1a receptor
Target Alias: Antidiuretic hormone receptor 1a; AVPR V1a; V1aR; Vascular/hepatic-type arginine vasopressin receptor
Product Type Specific: AVPR1A phosphosite-specific antibody
Antibody Code: PN787
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T378+S380
Protein UniProt: P37288
Protein SigNET: AVPR1A
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human AVPR1A
Antibody Immunogen Sequence: KED(pT)D(pS)MSC
Antibody Immunogen Description: Corresponds to amino acid residues K375 to S382. T378 and S380 phosphorylation is likely to be inhibitory to downstream signalling. The T378 phosphorylation has not been detected by mass spectrometry and the S380 site has been reported only once in PhosphoSite Plus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.55 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse | Platypus | Chicken.
Scientific Background: AVPR1A (Vasopressin V1a receptor) is a 7-transmembrane G protein-coupled receptor (7TM GPCR) that belongs to the G protein-coupled receptor 1 family, Vasopressin/oxytocin receptor subfamily. It is a receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins (e.g., Gaq, Ga11) which activate a phosphatidyl-inositol-calcium second messenger system. It has been linked to the manifestation of social behaviors, including affiliation and attachment. It regulates cardiovascular homeostasis, cell proliferation, and hepatic glycogenolysis. It is highly expressed in vascular smooth muscle, liver, and various regions of the brain. Differences in regional receptor expression in the brain as well as differences in social behavior may result from a highly variable repetitive sequence in the 5' flanking region of AVPR1A. One such allelic variant has been linked to autism. AVPR1A is linked to hypertension (via vasopressin-induced vasoconstriction) and considered a pharmacological target for social anxiety and cognitive disorders. This description may include information annotated by UniProt and/or Google AI.