Product Name:
AGTR1-pS335+pT336+pS338
Product Number:
ab-pn785
Target Full Name: Type-1 angiotensin II receptor
Target Alias: AT1AR;AT1BR;Angiotensin II type-1 receptor
Product Type Specific: AGTR1 phosphosite-specific antibody
Antibody Code: PN785
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S335+T336+S338
Protein UniProt: P30556
Protein SigNET: AGTR1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human AGTR1
Antibody Immunogen Sequence: TKM(pS)(pT)L(pS)YRP(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues T332 to P341. S335, T336 and S338 phosphorylation is likely to be inhibitory to downstream signalling, although there are resports of stimulated activity. This is likely targeted by a GRK family kinase.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.9 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rat | Mouse | Platypus | Chicken | Frog.
Scientific Background: AGTR1 (Type-1 angiotensin II receptor B) is a 7-transmembrane G protein-coupled receptor (7TM GPCR)belongs to the G protein-coupled receptor 1 family. Its ligand is angiotensin II, a vasoconstricting peptide, which acts as a key regulator of blood pressure and sodium retention by the kidney. The ligand-activated AGTR1 couples to G-alpha proteins G(q) (GNAQ, GNA11, GNA14 or GNA15) and stimulates phospholipase C to indirectly increase the cytosolic Ca2+ concentrations, which in turn triggers cellular responses such as stimulation of protein kinase C-alpha and beta isoforms. Mutations can cause renal tubular dysgenesis, a severe disorder of kidney development. It is a key target for antihypertensive medications such as Losartan, Valsartan, and Candesartan, which act as AT1 receptor blockers. This description may include information annotated by UniProt and/or Google AI.