Product Name:
ADRB2-pS355+pS356
Product Number:
ab-pn784
Target Full Name: Beta-2 adrenergic receptor
Target Alias: ADRB2R; ADRBR; Adrenergic receptor, beta-2; Adrenergic, beta-2-, receptor, surface; B2AR; BAR
Product Type Specific: ADRB2 phosphosite-specific antibody
Antibody Code: PN784
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S355+S356
Protein UniProt: P07550
Protein SigNET: ADRB2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human ADRB2
Antibody Immunogen Sequence: CGNGY(pS)(pS)NGN
Antibody Immunogen Description: Corresponds to amino acid residues G351 to N359. S355 and S356 phosphorylation is inhibitory to some downstream signalling by promoting desensitization, binding to beta-arrestin (ARRB2) and receptor internalization and degradation. ADRB2 is known to be phosphorylated at this site in vitro by BARK1 (GRK2, ADRBK1), GRK3, and GPRK5 (GRK5).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.66 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse.
Scientific Background: ADRB2 (beta-2 adrenergic receptor) is a 7-transmembrane G protein-coupled receptor (7TM GPCR) for catecholamines that couples to both G(s) and G(i) proteins, activating bifurcated signalling pathways. It belongs to the G protein-coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily. It binds epinephrine (Epi) with an approximately 30-fold greater affinity than norepinephrine (NE). Both NE and Epi promote coupling to G(s)/PKA pathway to regulate myocyte contraction rate. Epi also promotes ADRB2 coupling to G(i) proteins to exert cardioprotective effects especially in the conditions of hypoxia and oxidative stress through the G(i)/PI3K/Akt signalling pathway. In the heart, Epi- and NE-activated ADRB2 are thought to induced rapid and slow cardiomyocyte contraction rates, respectively. ADRB2-G(s) signalling delivers proapoptotic signals in cardiomyocytes, whereas G(i)-mediated survival effects predominate. ADRB2 also transduces signals independently of PKA to regulate cellular pH by modulating Na+/H+ exchanger SLC9A3 function. This description may include information annotated by UniProt and/or Google AI.