Product Name:
VHL-pS111
Product Number:
ab-pn783
Target Full Name: Von Hippel-Lindau disease tumor suppressor
Target Alias: Protein G7;pVHL
Product Type Specific: VHL phosphosite-specific antibody
Antibody Code: PN783
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S111
Protein UniProt: P40337
Protein SigNET: VHL
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human VHL
Antibody Immunogen Sequence: RIH(pS)YRG(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues R108 to G114. S111 phosphorylation induces interaction with Tip60 and p300. This is a minor site of in vivo phosphorylation of VHL based on ≥1 mass spectrometry reports recorded in PhosphoSitePlus. VHL is known to be phosphorylated at this site in vitro by Chk2.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Scientific Background: VHL (von Hippel-Lindau) protein is a crucial tumour suppressor in the VHL family. It functions as a target recruitment subunit in the E3 ubiquitin ligase complex, and regulates oxygen sensing and cell proliferation. It recruits hydroxylated hypoxia-inducible factor (HIF-1-alpha) for proteasomal degradation under normal oxygen levels, preventing tumour growth. The VHL protein helps stop cells from growing and dividing too rapidly, serving as a critical "gatekeeper" in cancer prevention. VHL acts as the substrate-recognition subunit of the VCB-CUL2 complex (comprising VHL, Elongin B, Elongin C, and Cullin-2). By tagging HIF-1 for degradation in oxygen-rich environments, it prevents improper angiogenesis (blood vessel formation) and metabolic reprogramming. VHL also plays roles in other cellular processes, including extracellular matrix formation, senescence regulation, and cilia formation. It is expressed in the adult and fetal brain and kidney. When the VHL gene is mutated or lost, VHL protein function is lost, leading to the accumulation of HIF, which drives excessive, uncontrolled cell growth and tumour formation. Inactivation of the VHL gene causes VHL syndrome, a hereditary disease, and is the primary driver of most sporadic, clear-cell renal cell carcinomas. This description may include information annotated by UniProt and/or Google AI.