Product Name:
UGDH-pY352
Product Number:
ab-pn782
Target Full Name: UDP-glucose 6-dehydrogenase
Target Alias: GDH; UDPGDH; UDP-Glc dehydrogenase; UDP-GlcDH; UDP-glucose dehydrogenase; UGD; Uridine diphospho-glucose dehydrogenase
Product Type Specific: UGDH phosphosite-specific antibody
Antibody Code: PN782
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y352
Protein UniProt: O60701
Protein SigNET: UGDH
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human UGDH
Antibody Immunogen Sequence: SSI(pY)ISK(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues S349 to K355. The effect of Y352 phosphorylation is unclear, but it is the major site of UGDH phosphorylation by mass spectometry studies (≥63 reports recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish | Sea urchin.
Scientific Background: UDP-glucose dehydrogenase (UGDH) is a cytosolic and nuclear hexameric enzyme that belongs to the UDP-glucose/GDP-mannose dehydrogenase family. It converts UDP-glucose to UDP-glucuronic acid (UDP-GlcUA) using NAD+, and it is important for producing extracellular matrix components like hyaluronan. It plays a vital role in glycosaminoglycan synthesis, xenobiotic metabolism, and cancer progression. It catalyzes the two-step oxidation of UDP-glucose to UDP-glucuronic acid, which is a precursor for glycosaminoglycans (hyaluronan, chondroitin sulfate). As a homohexamer (dimer-of-trimers) it undergoes conformational changes during catalysis. It enzymatic activity is regulated by allosteric feedback, such as by UDP-xylose. It is expressed in heart, placenta, liver, pancreas, spleen, thymus, prostate, ovary, small intestine and colon. Mutations in the UGDH gene are linked to a severe form of developmental epileptic encephalopathy. UGDH is also linked to tumour growth and metastasis. Its upregulation is associated with poor prognosis in various cancers (e.g., glioblastoma), while reduced expression is associated with cartilage degeneration (osteoarthritis). This description may include information annotated by UniProt and/or Google AI.