Product Name:
TPH1-pT225+pS228
Product Number:
ab-pn780
Target Full Name: Tryptophan 5-hydroxylase 1
Target Alias: ALS2CR3; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 3 protein; CALS-C; GRIF1; GRIF-1; KIAA0549; OIP98; Trafficking kinesin-binding 2; Trafficking protein, kinesin binding 2
Product Type Specific: TPH1 phosphosite-specific antibody
Antibody Code: PN780
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T225+S228
Protein UniProt: P17752
Protein SigNET: TPH1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human TPH1
Antibody Immunogen Sequence: KER(pT)GF(pS)IRP(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues K222 to P231. The effects of T225 and S228 phosphorylation are unclear. These are minor sites of in vivo phosphorylation of TPH1 with only 1 mass spectrometry report for each site in PhosphoSitePlus. However, there are very few phosphosites in this protein and they are only reported once.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish |Tiger blowfish | Fruit fly | Nematode worm.
Scientific Background: TPH1 (Tryptophan Hydroxylase 1) is a 50.9 kDa rate-limiting enzyme that catalyzes the crucial first and rate limiting step in the biosynthesis of peripheral serotonin, which is later made into melatonin in the pineal gland. As a Fe2+-dependent homotetramer, this oxidoreductase specifically converts L-tryptophan to 5-hydroxy-L-tryptophan (5-HTP) in the reaction: (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + L-tryptophan + O2 = 5-hydroxy-L-tryptophan + (4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin. It belongs to the biopterin-dependent aromatic amino acid hydroxylase family. While TPH1 is responsible for peripheral serotonin production, its homolog, TPH2, is responsible for central serotonin synthesis in the brain. TPH1 is primarily expressed in the gut, pineal gland, and mast cells, and regulates serotonin levels for various physiological functions, including GI motility and immune response. Isoform 1 is more widely expressed than isoform 2. Mutations or polymorphisms (such as A218C) in the TPH1 gene are linked to psychiatric conditions like schizophrenia, bipolar disorder, suicidal behavior, and addiction. This description may include information annotated by UniProt and/or Google AI.