Product Name:
SETD2-pS2080+pS2082
Product Number:
ab-pn779
Target Full Name: Histone-lysine N-methyltransferase SETD2
Target Alias: FLJ23184; HIF-1; Huntingtin interacting protein 1; HYPB; KIAA1732; KMT3A; SET domain containing 2
Product Type Specific: SETD2 phosphosite-specific antibody
Antibody Code: PN779
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S2080+S2082
Protein UniProt: Q9BYW2
Protein SigNET: SETD2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human SETD2
Antibody Immunogen Sequence: RRS(pS)L(pS)PPSC
Antibody Immunogen Description: Corresponds to amino acid residues R2077 to S2085. The effects of S2080 and S2082 phosphorylation are unclear. These are the major sites of in vivo phosphorylation of SETD2 based on ≥73 and ≥71 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse.
Scientific Background: SETD2 (Histone-lysine N-methyltransferase SETD2; SET Domain Containing 2) is a crucial human histone methyltransferase that catalyzes the trimethylation of Lys-36 on histone H3 (H3K36me3). It specifically catalyzes the conversion of dimethylated to trimethylated Lys-36 on histone H3 (H3 K36me2 -> H3 K36me3). It belongs to the class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, SET2 subfamily. It also plays a role in DNA damage repair, specifically homologous recombination. It features a central SET domain (catalytic activity), a post-SET domain (stability), and an SRI domain that interacts with RNA polymerase II, linking methylation to active transcription. It plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. It acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction. It is required for DNA double-strand break repair in response to DNA damage: acts by mediating formation of H3K36me3, promoting recruitment of RAD51 and DNA repair via homologous recombination (HR). It acts as a tumour suppressor. H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A. H3K36me3 is also enhanced in intron-containing genes, indicating that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase. It is required during angiogenesis. It is needed for endoderm development by promoting embryonic stem cell differentiation toward endoderm. It acts by mediating formation of H3K36me3 in distal promoter regions of FGFR3, leading to regulate transcription initiation of FGFR3. In addition to histones, SETD2 also mediates methylation of other proteins, such as tubulins and STAT1. It trimethylates Lys-40 of alpha-tubulins such as TUBA1B (alpha-TubK40me3); alpha-TubK40me3 is required for normal mitosis and cytokinesis and may be a specific tag in cytoskeletal remodeling. Involved in interferon-alpha-induced antiviral defense by mediating both monomethylation of STAT1 at Lys-525 and catalyzing H3K36me3 on promoters of some interferon-stimulated genes (ISGs) to activate gene transcription. It acts as a tumour suppressor by regulating gene transcription, DNA repair, alternative splicing, and genomic stability. It is a key player in maintaining chromatin integrity and regulating cellular, particularly in immune cell, functions. As the sole enzyme responsible for H3K36me3, its dysfunction is linked to various cancers. Loss-of-function mutations are found in numerous cancers, including clear cell renal cell carcinoma, leukemia, and gastrointestinal cancers. Its dysfunction is associated with developmental disorders, such as Luscan-Lumish syndrome. This description may include information annotated by UniProt and/or Google AI.