Product Name:
PPFIBP1-pS599+pS601
Product Number:
ab-pn777
Target Full Name: Liprin-beta-1
Target Alias: HSGT2; KIAA1230; L2; LIPB1; Liprin beta 1; Protein tyrosine phosphatase receptor type f polypeptide-interacting protein binding protein 1; Protein tyrosine phosphatase receptor type f polypeptide-interacting protein-binding protein 1; PTPRF interacting protein, binding protein 1; PTPRF-interacting protein binding protein 1; PTPRF-interacting protein-binding protein 1; SGT2
Product Type Specific: PPFIBP1 phosphosite-specific antibody
Antibody Code: PN777
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S599+S601
Protein UniProt: Q86W92
Protein SigNET: PPFIBP1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PPFIBP1
Antibody Immunogen Sequence: LRR(pS)Q(pS)TTF(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues L596 to F604. The effects of S599 and S601 phosphorylation are unclear. These are major sites of in vivo phosphorylation based on ≥74 and ≥223 mass spectrometry reports recorded, respectively, in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Frog.
Scientific Background: PPFIBP1 (PPFIA-binding protein 1; liprin-beta-1) is a scaffold protein that belongs to the liprin family, Liprin-beta subfamily. It is crucial for neuronal development, synaptic formation, and cell motility, often acting via interactions with liprin-alpha proteins and S100A4. It acts as a scaffolding and regulatory protein, organizing signaling complexes at membrane protrusions. It is involved in regulating focal adhesion disassembly and interacts with LAR-family tyrosine phosphatases. It features SAM (sterile alpha motif) domains that are crucial for protein-protein interactions; disruption of these domains can lead to structural instability and functional loss. It is widely expressed, but missing from liver. Bi-allelic loss-of-function variants in PPFIBP1 result in a syndrome involving severe intellectual disability, refractory epilepsy, progressive microcephaly, spasticity, and brain abnormalities like leukoencephalopathy. Overexpression of PPFIBP1 promotes tumour invasiveness and metastasis in glioma and melanoma, often by activating FAK/Src/JNK signaling pathways and increasing matrix metalloproteinase-2 (MMP-2) expression. This description may include information annotated by UniProt and/or Google AI.