Product Name:
PLCG1-pY771+pY775
Product Number:
ab-pn774
Target Full Name: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2
Target Alias: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; NCKAP3; Phospholipase C, gamma 1; Phospholipase C-gamma-1; PLC1; PLC148; PLC-148; PLCG-1; PLCgamma1; PLC-gamma1; PLC-II
Product Type Specific: PLCG1 phosphosite-specific antibody
Antibody Code: PN774
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y771+Y775
Protein UniProt: P19174
Protein SigNET: PLCG1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PLCG1
Antibody Immunogen Sequence: EPD(pY)GAL(pY)EGR(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues E768 to R778. Y771 phosphorylation inhibits enzyme activity and the effect of Y775 phosphorylation is unclear. These are major in vivo phosphorylation site sin PLCG1 (≥587 and >374 reports, respectively, from high throughput mass spectrometry studies recorded in PhosphoSitePlus). PLCG1 is known to be phosphorylated in vitro at Y551 by Abl1, EGFR, FGFR2, and Syk.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse.
Related Product 1: PLCB3 S1105 phosphorylation antibody (Cat. No.: AB-PN719)
Related Product 2: PLCE1 S1096+T1100 phosphorylation antibody (Cat. No.: AB-PN721)
Related Product 3: PLCG1 Y783 phosphorylation antibody (Cat. No.: AB-PN530)
Related Product 4: PLCG1 Y977 phosphorylation antibody (Cat. No.: AB-PN722)
Related Product 5: PLCG2 Y1217 phosphorylation antibody (Cat. No.: AB-PN776)
Related Product 6: PLCG2 Y753 phosphorylation antibody (Cat. No.: AB-PN723)
Related Product 7: PLCG2 Y759 phosphorylation antibody (Cat. No.: AB-PN531)
Related Product 8: PLCG2 Y759 phosphorylation antibody (Cat. No.: AB-PN775)
Scientific Background: PLCG1 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1) mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). It plays an important role in the regulation of intracellular signaling cascades, regulating crucial cellular processes including for growth, proliferation, migration, and apoptosis. It becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. It also functions in actin reorganization. It acts as a calcium-dependent phospholipase C, binding to calcium ions and regulatory proteins. Contains multiple domains including catalytic X and Y domains, SH2, and SH3 domains, which mediate interaction with upstream tyrosine kinases and downstream effectors. Overexpression or mutations are linked to various human cancers, including T-cell malignancies, angiosarcoma, and gliomas, where it promotes tumour invasion and survival. PLCG1 dysfunction can also lead to other pathological conditions like autoimmunity. This description may include information annotated by UniProt and/or Google AI.