Product Name:
PKM2-pY105
Product Number:
ab-pn772
Target Full Name: Pyruvate kinase isozymes M1/M2
Target Alias: CTHBP; Cytosolic thyroid hormone-binding protein; EC 2.7.1.40; KPYM; PKM; Pyruvate kinase 2/3; Pyruvate kinase M; Pyruvate kinase muscle isozyme; Pyruvate kinase, M2 isozyme
Product Type Specific: PKM2 phosphosite-specific antibody
Antibody Code: PN772
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y105
Protein UniProt: P14618
Protein SigNET: PKM2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PKM2
Antibody Immunogen Sequence: PIL(pY)RPVAC
Antibody Immunogen Description: Corresponds to amino acid residues P102 to A109 . Y105 phosphorylation inhibits phosphotransferase activity, although there is one report of activation), and inhibits interaction with PKM and TRIM35. This is one of the prominent in vivo phosphorylation sites in PKM2 (≥445 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). PKM2 is known to be phosphorylated at this site in vitro by FGFR1, and NPM-ALK.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.6 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Zebra fish.
Related Product 1: PKM2 S37 phosphorylation antibody (Cat. No.: AB-PN718)
Related Product 2: PKM2 Y148 phosphorylation antibody (Cat. No.: AB-PN717)
Related Product 3: PKM2 Y370 phosphorylation antibody (Cat. No.: AB-PN773)
Related Product 4: PKM2 Y390 phosphorylation antibody (Cat. No.: AB-PN529)
Scientific Background: PKM2 functions as a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, and generates ATP. It stimulates POU5F1-mediated transcriptional activation. It appears to plays a general role in caspase independent cell death of tumour cells. The ratio between its highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is thought to be important for tumour cell proliferation and survival. This description may include information annotated by UniProt and/or Google AI.