Product Name:
PCK1-pS118
Product Number:
ab-pn768
Target Full Name: Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Target Alias: PEPCK1; PEPCK-C; Phosphoenolpyruvate carboxykinase 1 (soluble); Phosphoenolpyruvate carboxylase; PPCKC
Product Type Specific: PCK1 phosphosite-specific antibody
Antibody Code: PN768
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S118
Protein UniProt: P35558
Protein SigNET: PCK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PCK1
Antibody Immunogen Sequence: CGRWM(pS)EED
Antibody Immunogen Description: Corresponds to amino acid residues G114 to D121. The effect of S118 phosphorylation is unclear. This is one of the major site of in vivo phosphorylation of PBRM1 by mass spectrometry (≥7 reports recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Chicken.
Scientific Background: PCK1 (Phosphoenolpyruvate carboxykinase, cytosolic [GTP]) is a key cytoplasmic enzyme that converts oxaloacetate to phosphoenolpyruvate (PEP) in gluconeogenesis. It acts as a rate-limiting step in gluconeogenesis, essential for generating glucose from non-carbohydrate precursors. It functions as a metabolic sensor for nutrient levels, primarily regulates glucose homeostasis in the liver, kidneys and adipocytes. It regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle. At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate. It also functions in amino acid metabolism, TCA cycle anaplerosis. Its expression is regulated by hormones like insulin, glucagon, and glucocorticoids, and it is degraded via p300-mediated acetylation. It acts as a tumour suppressor or promoter depending on the cancer context. When phosphorylated by AKT, it can function as a protein kinase to support cancer lipogenesis. This description may include information annotated by UniProt and/or Google AI.