Product Name:
ODC1-pT93
Product Number:
ab-pn766
Target Full Name: Ornithine decarboxylase
Target Alias: ODC
Product Type Specific: ODC1 phosphosite-specific antibody
Antibody Code: PN766
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T93
Protein UniProt: P11926
Protein SigNET: ODC1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human ODC1
Antibody Immunogen Sequence: CASK(pT)EIQL
Antibody Immunogen Description: Corresponds to amino acid residues A90 to L97. The effect of ODC1 T93 phosphorylation is unclear. This is one of the few in vivo phosphorylation sites of ODC1 that has been identified by mass spectrometry (≥2 reports recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish.
Scientific Background: ODC1 (Ornithine decarboxylase 1) is a 48-53.5 kDa protein that belongs to the Orn/Lys/Arg decarboxylase class-II family. It is the first and rate-limiting enzyme in polyamine biosynthesis with its conversion of ornithine to putrescine with the enzyme reaction: L-ornithine + H+ = putrescine + CO2. Polyamines, such as putrescine, spermidine, and spermine, are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication to apoptosis. It is highly expressed in rapidly growing tissues. Its activity is tightly regulated by ubiquitin-dependent and independent degradation. It is inhibited by S-nitrosylation. It is inhibited by antizymes (AZs) OAZ1, OAZ2 and OAZ3 in response to polyamine levels. AZs inhibit the assembly of the functional homodimer by binding to ODC monomers. Additionally, OAZ1 targets ODC monomers for ubiquitin-independent proteolytic destruction by the 26S proteasome. At least 13 ubiquitinylated sites have been identified in ODC1. It is often upregulated in cancers (e.g., neuroblastoma, prostate, colon) due to oncogenes like MYCN/c-Myc. It is down-regulated in response to enterovirus 71 (EV71) infection. This description may include information annotated by UniProt and/or Google AI.