Product Name:
NOS1-pS746
Product Number:
ab-pn762
Target Full Name: Nitric oxide synthase, brain
Target Alias: BNOS; Constitutive NOS; NC-NOS; Neuronal NOS; Nitric oxide synthase 1 (neuronal); Nitric-oxide synthase, brain; N-NOS; NOS; NOS, type I; NOS1
Product Type Specific: NOS1 phosphosite-specific antibody
Antibody Code: PN762
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S746
Protein UniProt: P29475
Protein SigNET: NOS1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human NOS1
Antibody Immunogen Sequence: CAVKF(pS)AKL
Antibody Immunogen Description: Corresponds to amino acid residues A742 to L749. S746 phosphorylation inhibits the enzymatic activity of NOS1. Phosphorylation of this site in NOS1 has not been reported by mass spectrometry. This human phosphosite is highly conserved in vertebrates and also found in the fruit fly. NOS1 is known to be phosphorylated at this site in vitro by CaMK1a.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Frog | Zebra fish | Fruit fly.
Related Product 1: NOS2 S745 phosphorylation antibody (Cat. No.: AB-PN711)
Related Product 2: NOS3 T1175+S1177 phosphorylation antibody (Cat. No.: AB-PN712)
Related Product 3: NOS2 Y151 phosphorylation antibody (Cat. No.: AB-PN763)
Scientific Background: NOS1 (Neuronal Nitric Oxide Synthase 1 or nNOS) is a Ca2+/calmodulin-dependent enzyme that belongs to the NOS family. It synthesizes nitric oxide (NO) from L-arginine with the reaction: 2 L-arginine + 3 NADPH + 4 O2 + H+ = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. It also requires cofactors including FAD, FMN, NADPH, and tetrahydrobiopterin. NO acts as a crucial neurotransmitter in the brain and peripheral nervous system, and mediates neurotransmission, neurodevelopment, cerebral blood flow regulation, and peripheral nerve-mediated relaxation (e.g., peristalsis). It is vital for synaptic plasticity, learning, memory, and smooth muscle regulation. It is thought to have nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. It is highly expressed in neurons in the central nervous system (cerebrum, hippocampus, cerebellum) and beneath the sarcolemma of skeletal muscle. Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina, and not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland. Dysregulation is linked to neurodegenerative diseases (Alzheimer's, Parkinson's), psychiatric disorders (schizophrenia, bipolar), and gastrointestinal motility disorders (achalasia, pyloric stenosis). This description may include information annotated by UniProt and/or Google AI.