Product Name:
NF1-pY2577
Product Number:
ab-pn761
Target Full Name: Neurofibromin GTPase
Target Alias: Neurofibromatosis; Neurofibromatosis-related protein NF-1; Neurofibromin 1; Neurofibromin truncated; Von Recklinghausen disease; Watson disease
Product Type Specific: NF1 phosphosite-specific antibody
Antibody Code: PN761
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y2577
Protein UniProt: P21359
Protein SigNET: NF1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human NF1
Antibody Immunogen Sequence: CAETD(pY)EME
Antibody Immunogen Description: Corresponds to amino acid residues A2573 to E2580. The effect of Y2577 phosphorylation is unclear. This is the major in vivo phosphorylation site in NF1 (≥103 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is highly conserved in vertebrates and also found in fish.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.2 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus | Chicken.
Scientific Background: NF1 (Neurofibromin) is a large 250-280 kDa cytoplasmic protein acting as a tumour suppressor by regulating cell growth, division, and differentiation. It primarily functions as a GTPase-activating protein (GAP) that inactivates the Ras signalling pathway. It functions as a negative regulator of the RAS/MAPK pathway, accelerating the hydrolysis of Ras-bound GTP, which keeps cells from uncontrolled proliferation. It is a large, multi-domain protein consisting of 2,818 amino acids and functions as a homodimer. It exists in both auto-inhibited (closed) and active (open) conformations. It features a specific domain (GRD) that interacts with Ras, stimulating the hydrolysis of GTP to GDP, turning "on" Ras signals "off". It is involved in additional signaling pathways, including Akt/mTOR and cAMP/PKA, affecting cytoskeleton dynamics and neural development. Besides Ras regulation, it has been shown to interact with proteins like HCN1 to regulate neuronal excitability. While widely expressed, especially in brain, peripheral nerve, lung, colon and muscle, it is most highly concentrated in neurons, Schwann cells, and oligodendrocytes. Loss-of-function mutations lead to neurofibromatosis type 1, characterized by tumours (neurofibromas), café-au-lait spots, and increased risk of malignancies like peripheral nerve sheath tumours. This description may include information annotated by UniProt and/or Google AI.