Product Name:
MPL-pY591
Product Number:
ab-pn760
Target Full Name: Thrombopoietin receptor
Target Alias: CD110; CD110 antigen; C-mpl; MPL; Myeloproliferative leukemia protein; Myeloproliferative leukemia virus oncogene; Myeloproliferative leukemia virus oncogene, thrombopoietin receptor; TPOR; TPO-R
Product Type Specific: MPL phosphosite-specific antibody
Antibody Code: PN760
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y591
Protein UniProt: P40238
Protein SigNET: MPL
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human MPL
Antibody Immunogen Sequence: CAQMD(pY)RRL
Antibody Immunogen Description: Corresponds to amino acid residues A587 to L594. The effect of Y591 phosphorylation is unclear. This is the major in vivo phosphorylation site in MPL (≥17 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is conserved in mammals, and found in the mouse.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.2 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Scientific Background: MPL (Thrombopoietin receptor, TPOR) is a 635-amino acid cytokine receptor that is a Class I Cytokine Receptor, Type 1 subfamily. It features an extracellular ligand-binding domain (composed of two cytokine receptor modules), a transmembrane domain, and a cytoplasmic domain. It plays critical role in the regulation of platelet production (thrombopoiesis) and the self-renewal of hematopoietic stem cells. It is primarily expressed on the surface of megakaryocyte progenitors. When its ligand, thrombopoietin (TPO), binds to MPL, the receptor dimerizes and activates the JAK/STAT pathway, promoting the proliferation, survival, and differentiation of megakaryocytes into platelets. It is essential for the maintenance and expansion of hematopoietic stem cells in the bone marrow. TPO induces rapid tyrosine phosphorylation and activates JAK2, which then triggers downstream signaling pathways with STAT5, PI3K, and ERK1/2 MAPK. It is expressed at a low level in a large number of cells of hematopoietic origin. Isoform 1 and isoform 2 are always found to be coexpressed. Acquired mutations in the MPL gene (often at positions W515 or S505) lead to a constitutively active receptor (ligand-independent signaling), resulting in the overproduction of platelets and abnormal megakaryocytes. This is associated with essential thrombocythemia (ET) and primary myelofibrosis (PMF). Congenital amegakaryocytic thrombocytopenia (CAMT) can arise from inherited mutations that cause loss of MPL function, which lead to severe thrombocytopenia (low platelets) and bone marrow failure. This description may include information annotated by UniProt and/or Google AI.