Product Name:
MAT1A-pT341
Product Number:
ab-pn759
Target Full Name: S-adenosylmethionine synthase isoform type-1
Target Alias: Methionine adenosyltransferase 1;Methionine adenosyltransferase I/III
Product Type Specific: MAT1A phosphosite-specific antibody
Antibody Code: PN759
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T341
Protein UniProt: Q00266
Protein SigNET: MAT1A
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human MAT1A
Antibody Immunogen Sequence: SQK(pT)ERE(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues S338 to E344. The effect of T341 phosphorylation is unclear. Phosphorylation at this site in MAT1A has only been reported in one mass spectrometry study. This human phosphosite is highly conserved in vertebrates and also found in yeast. MAT1A is known to be phosphorylated at this site in vitro by PKCa (PRKCA).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Chicken | Frog | Zebra fish | Sea urchin .
Scientific Background: MAT1A (Methionine Adenosyltransferase 1A) encodes a crucial liver-specific enzyme that catalyzes the synthesis of S-adenosylmethionine (SAMe) from methionine and ATP. It catalyzes the reaction: L-methionine + ATP + H2O =. S-adenosyl-L-methionine + phosphate + diphosphate. As the main methyl donor in liver cells, this protein exists as tetramers (MAT I) or dimers (MAT III). SAMe is essential for transmethylation reactions, including DNA regulation and neurochemistry. It is exclusively expressed in adult liver and is essential for liver homeostasis; it helps regulate cell proliferation. It forms MAT I (homotetramer) and MAT III (homodimer). Deficiencies in MAT1A causes persistent elevated blood methionine levels (hypermethioninemia). A decrease in MAT1A is often accompanied by an increase in MAT2A (known as the MAT1A/MAT2A switch), and is associated with liver cirrhosis, fibrosis, and hepatocellular carcinoma. This description may include information annotated by UniProt and/or Google AI.