Product Name:
IKKg-pS377
Product Number:
ab-pn758
Target Full Name: I-kappa-B kinase gamma/NF-kappa-B essential modulator
Target Alias: FIP3; FIP-3; I-kappa-B kinase gamma; IkB kinase gamma subunit; IkB kinase-associated protein 1; IKBKG; IKKAP1; IKKG; Inhibitor of nuclear factor kappa-B kinase gamma subunit; MFIP-3; NEMO; NF-kappaB essential modifier; NF-kappaB essential modulator
Product Type Specific: IKKg phosphosite-specific antibody
Antibody Code: PN758
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S377
Protein UniProt: Q9Y6K9
Protein SigNET: IKKg
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human IKKg
Antibody Immunogen Sequence: AYLS(pS)PLAC
Antibody Immunogen Description: Corresponds to amino acid residues A373 to A380. S377 phosphorylation inhibits enzymatic activity. This is the major in vivo phosphorylation sites in IKKg (≥56 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is not conserved in vertebrates, but is found in the chimpanzee. IKKg is known to be phosphorylated at this site in vitro by ERK1 (MAPK3).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee.
Related Product 1: IKKSelectide kinase substrate peptide (Cat. No.: PE-01BHN95)
Related Product 2: IKKa-pT179+pS180 phosphosite-specific antibody (Cat. No.: AB-PK659)
Related Product 3: IKKb-BCT pan-specific antibody (Cat. No.: AB-NK076-5)
Related Product 4: IKKb-NT pan-specific antibody (Cat. No.: AB-NK076-6)
Related Product 5: IKKe-pS172 phosphosite-specific antibody (Cat. No.: AB-PK660)
Scientific Background: IKKg (IkB kinase-gamma; NFkB Essential Modulator; NEMO) IKKAP1) ) is a 48-50 kDa regulatory, non-catalytic scaffolding subunit of the IKK core complex that phosphorylates inhibitors (IkB) of NF-kappa-B. It is necessary for the activation of IKKb. IKK phosphorylation of IkB faciltates dissociation of the inhibitor/NF-kappa-B complex, the degradation of the inhibitor, and allows NFkB to enter the nucleus and trigger gene expression. IKKg contains domains that bind to polyubiquitinated upstream signalling molecules (such as RIP1 or TRAF6), which is essential for recruiting the IKK complex to sites of activation. Its binding to scaffolding polyubiquitin plays a key role in IKK activation in multiple signaling receptor pathways. It can recognize and bind both 'Lys-63'-linked and linear polyubiquitin upon cell stimulation, with a much higher affinity for linear polyubiquitin. It has been implicated in NFkB-mediated protection from cytokine toxicity. It is necessary for viral activation of IRF3. It is also involved in TLR3- and IFIH1-mediated antiviral innate response, which requires requires 'Lys-27'-linked polyubiquitination of IKKg. It also serves as a docking site for negative regulators such as A20 and CYLD, aiding in the downregulation of their signalling pathways. It interacts with TRAF6, RIPK1, and TANK, especially under stimulation or DNA damage. In the nucleus, IKKg plays an IKK-independent role, where it is involved in the response to DNA damage by facilitating the nuclear export of the complex upon phosphorylation by ATM. It features containing two coiled-coil domains (CC1, CC2), a leucine zipper (LZ), and a C-terminal zinc finger domain. It is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Mutations in IKKg are associated with several serious genetic disorders, including Incontinentia Pigmenti (usually lethal in males and causes skin, hair, teeth, and CNS abnormalities in females), X-linked Hypohidrotic Ectodermal Dysplasia with Immune Deficiency, and NEMO Deficiency Syndrome ( a rare, often fatal syndrome due to complete lack of NF-kB activation). This description may include information annotated by UniProt and/or Google AI.