Product Name:
IDH1-pY391
Product Number:
ab-pn757
Target Full Name: Isocitrate dehydrogenase [NADP] cytoplasmic
Target Alias: Cytosolic NADP-isocitrate dehydrogenase; IDH; IDHC; IDP; Isocitrate dehydrogenase 1 (NADP+) soluble; NADP+-specific ICDH; Oxalosuccinate decarboxylase; PICD
Product Type Specific: IDH1 phosphosite-specific antibody
Antibody Code: PN757
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y391
Protein UniProt: O75874
Protein SigNET: IDH1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human IDH1
Antibody Immunogen Sequence: RSD(pY)LNT(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues R388 to T394. The effect of Y391 phosphorylation is unclear. This is the major in vivo phosphorylation site in IDH1 (≥34 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is highly conserved in vertebrates and also found in sea urchin. IDH1 is known to be phosphorylated at this site in vitro by FLT3.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.8 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Rat | Mouse | Platypus | Chicken | Frog | Nematode worm | Sea urchin.
Related Product 1: IDH1 T75+T77 phosphorylation antibody (Cat. No.: AB-PN706)
Scientific Background: IDH1 (Isocitrate Dehydrogenase 1) is a dimeric, NADP+-dependent enzyme that belongs to the isocitrate and isopropylmalate dehydrogenases family. It is located in the cytoplasm and peroxisomes that catalyzes the reversible oxidative decarboxylation of isocitrate into alpha-ketoglutarate (alpha-KG) and CO2, generating NADPH. The enzyme reaction is: D-threo-isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. This reaction is crucial for cellular metabolism (e.g., for phytanoyl-CoA dioxygenase), and NADPH production is needed for lipid synthesis and defending cells against reactive oxygen species. It also likely acts as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency. It is distinct from the mitochondrial IDH2 and IDH3 enzymes, although they perform similar reactions. IDH1 is predominantly located in the cytoplasm, with some presence in peroxisomes. IDH1 mutations are common in low-grade gliomas, secondary glioblastomas, and acute myeloid leukemia (AML). Mutations, particularly at Arg-132 (R132), inhibit its normal function and instead convert alpha-ketoglutarate into the oncometabolite 2-hydroxyglutarate (2-HG). The accumulation of 2-HG leads to extensive epigenetic changes, including inhibition of histone demethylases and DNA hydroxymethylases, leading to impaired cell differentiation and tumorigenesis. This description may include information annotated by UniProt and/or Google AI.