Product Name:
GCLC-pS5+pS8
Product Number:
ab-pn752
Target Full Name: Glutamate--cysteine ligase catalytic subunit
Target Alias: Gamma-ECS; Gamma-glutamylcysteine synthetase; GCS; GCS heavy chain; GLCL; GLCLC; Glutamate-cysteine ligase catalytic; Glutamate-cysteine ligase, catalytic; Glutamate-cysteine ligase, catalytic subunit; GSH1
Product Type Specific: GCLC phosphosite-specific antibody
Antibody Code: PN752
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S5+S8
Protein UniProt: P48506
Protein SigNET: GCLC
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human GCLC
Antibody Immunogen Sequence: GLL(pS)QG(pS)PLS(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues G2 to S11. The effects of S5 and S8 phosphorylation are unclear. These are two of the major in vivo phosphorylation sites in GCLC (≥12 and >15 reports, respectively, by mass spectrometry recorded in PhosphoSitePlus). These human phosphosites are highly conserved in vertebrates and also found in yeast.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.4 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Frog | Zebra fish | Fruit fly | Nematode worm.
Scientific Background: GCLC (Glutamate--cysteine ligase catalytic subunit) is a ligase that belongs to the glutamate--cysteine ligase type 3 family, and and is essential for cellular redox homeostasis. It catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis. It catalyzes the reaction: L-cysteine + L-glutamate + ATP = gamma-L-glutamyl-L-cysteine + ADP + phosphate + H+. It is the heavy, catalytic subunit of the heterodimeric glutamate-cysteine ligase protein, whereas GCLM (light subunit) increases its efficiency to produce glutathione, a critical antioxidant. Its activity is crucial for managing oxidative stress, often regulated by Nrf2 translocation, which is sensitive to metabolic changes and toxic insults. It is typically found in the cytoplasm and is important for maintaining cellular detoxification of ROS (reactive oxygen species). Mutations or deficiency in GCLC can cause hemolytic anemia and increase susceptibility to oxidative damage-related diseases, such as myocardial infarction. This description may include information annotated by UniProt and/or Google AI.