Product Name:
FBPase2-pY259
Product Number:
ab-pn751
Target Full Name: Fructose-1,6-bisphosphatase isoform 2
Target Alias: D-fructose-1,6-bisphosphate 1-phosphohydrolase 2; EC 3.1.3.11; F16P2; FBPase; Fructose-1,6-bisphosphatase 2; Hexosediphosphatase; Muscle fructose-bisphosphatase
Product Type Specific: FBPase phosphosite-specific antibody
Antibody Code: PN751
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y259
Protein UniProt: O00757
Protein SigNET: FBPase2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human FBPase2
Antibody Immunogen Sequence: RTLV(pY)GGI(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues R255 to I262. The effect of Y259 phosphorylation is unclear. Phosphorylation at this site in fructose-1,6-bisphosphatase isoform 2 has only been reported in a single mass spectrometry study. However, this human phosphosite is highly conserved in vertebrates and found in yeast.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.9 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Mouse | Platypus | Chicken | Frog | Zebra fish | Thale cress | Baker's yeast.
Related Product 1: FBPase S88 phosphorylation antibody (Cat. No.: AB-PN750)
Related Product 2: FBPase2 Y216 phosphorylation antibody (Cat. No.: AB-PN700)
Scientific Background: FBPase2 is a bifunctional enzyme, which plays a central role in regulating glycolysis and gluconeogenesis. It catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate (beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate) in the presence of divalent cations, and participates in glycogen synthesis from carbohydrate precursors, such as lactate. While PFK-2 synthesizes fructose-2,6-bisphosphate (Fru-2,6-P2), the FBPase-2 domain degrades it into fructose-6-phosphate and inorganic phosphate, thereby regulating the levels of this critical activator of glycolysis. Fructose 2,6-bisphosphate is a competitive inhibitor and FBPase is strongly inhibited by Ca2+. In many isoforms, phosphorylation by Protein Kinase A (PKA) or Protein Kinase B (Akt) inhibits the PFK-2 activity and activates the FBPase-2 activity. The enzyme exists as a homodimer (approx. 55 kDa per subunit). PFKFB2 (Cardiac/H-type) is highly expressed in the heart. The PFKFB2-encoded enzyme is crucial for regulating glucose metabolism, particularly in cardiac and adipose tissues. FBPase-2 activity plays a role in heart functioning, particularly regarding the control of glycolysis under hypoxia (low oxygen) conditions. The PFKFB2 gene is linked to the predisposition to schizophrenia. FBPase-2 activity is often inhibited in tumor cells (e.g., through AKT-dependent phosphorylation of PFKFB) to allow increased Fru-2,6-P2 levels, which fuels the rapid glycolysis known as the Warburg effect.This description may include information annotated by UniProt and/or Google AI.