Product Name:
FBPase-pS88
Product Number:
ab-pn750
Target Full Name: Fructose-1,6-bisphosphatase isoform 1
Target Alias: D-fructose-1,6-bisphosphate 1-phosphohydrolase; EC 3.1.3.11; F16P; F16P1; FBP; FBP1
Product Type Specific: FBPase phosphosite-specific antibody
Antibody Code: PN750
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S88
Protein UniProt: P09467
Protein SigNET: FBPase
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human FBPase
Antibody Immunogen Sequence: MLK(pS)SFATC
Antibody Immunogen Description: Corresponds to amino acid residues M85 to T92. S88 phosphorylation inhibits enzyme activity. Phosphorylation at this site in fructose-1,6-bisphosphatase isoform 1 has only been reported in a single mass spectrometry study. However, this human phosphosite is highly conserved in vertebrates and found in fish. FBPase is known to be phosphorylated at this site in vitro by ULK1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Related Product 1: FBPase2 Y216 phosphorylation antibody (Cat. No.: AB-PN700)
Related Product 2: FBPase2 Y259 phosphorylation antibody (Cat. No.: AB-PN751)
Scientific Background: FBPase (Fructose-1,6-bisphosphatase; FBP1) is a critical, rate-limiting cytosolic enzyme in gluconeogenesis. It catalyzes the irreversible hydrolysis of fructose-1,6-bisphosphate (F1,6BP) to fructose-6-phosphate (F6P) and inorganic phosphate. It plays a crucial role in maintaining blood glucose homeostasis. It plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. It appears to modulate glycerol gluconeogenesis in liver. Mammalian FBPase exists as a homotetramer, composed of four identical polypeptide chains (approx. 37 kDa each). These tetramers are composed of two intimate dimers, which oscillate between an active (R) and inactive (T) conformational state. In mammals, there are two main isoforms: FBPase-1 (liver/kidney, crucial for gluconeogenesis) and FBPase-2 (muscle). ATP acts as a positive regulator, and it is allosterically inhibited by adenosine monophosphate (AMP) and fructose-2,6-bisphosphate (F2,6BP), which stabilizes the inactive T-state. Phosphorylation of FBPase by protein kinase A (PKA) decreases its enzyme activity. This description may include information annotated by UniProt and/or Google AI.