Product Name:
FASN-pY45
Product Number:
ab-pn749
Target Full Name: Fatty acid synthase
Target Alias: [Acyl-carrier-protein] S-acetyltransferase; FAS; fatty acid synthase; SDR27X1; short chain dehydrogenase/reductase family 27X, member 1
Product Type Specific: FASN phosphosite-specific antibody
Antibody Code: PN749
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y45
Protein UniProt: P49327
Protein SigNET: FASN
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human FASN
Antibody Immunogen Sequence: AGL(pY)GLPRC
Antibody Immunogen Description: Corresponds to amino acid residues A42 to R490. The effect of Y45 phosphorylation is unclear. This is the major in vivo phosphorylation site in fatty acid synthetase (≥90 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is highly conserved in vertebrates and also found in the bee.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Chicken | Zebra fish | Fruit fly | Nematode worm.
Related Product 1: FASN S207 phosphorylation antibody (Cat. No.: AB-PN698)
Scientific Background: FASN (Fatty acid synthase) is a vital, homodimeric multi-enzyme protein (encoded by the FASN gene in humans) that catalyzes the de novo synthesis of long-chain fatty acids, primarily palmitate (C16:0), from acetyl-CoA, malonyl-CoA, and NADPH. It is a cytosolic, multifunctional enzyme with seven distinct catalytic activities, and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain. It catalyzes the reaction: acetyl-CoA + n malonyl-CoA + 2n NADPH + 2n H+ = a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP+. It is highly expressed in brain, lung, and liver. Elevated expression of FAS is linked to obesity, non-alcoholic fatty liver disease, and various cancers. This description may include information annotated by UniProt and/or Google AI.