Product Name:
EP300-pS2279
Product Number:
ab-pn748
Target Full Name: Histone acetyltransferase p300
Target Alias: 300 kd E1A-Binding protein gene; E1A binding protein p300; E1A-associated protein p300; Histone acetyltransferase p300; KAT3B; P300; P300 transcription coactivator homologue
Product Type Specific: EP300 phosphosite-specific antibody
Antibody Code: PN748
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S2279
Protein UniProt: Q09472
Protein SigNET: EP300
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human EP300
Antibody Immunogen Sequence: NPM(pS)PQQ(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues N2276 to Q2282. S2279 phosphorylation stimulates enzyme activity. Phosphorylation at this site in EP300 has only been reported in a single mass spectrometry study. However, this human phosphosite is highly conserved in vertebrates and is found in fish. EP300 is known to be phosphorylated at this site in vitro by ERK2 (MAPK1).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.45 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse | Chicken | Frog | Zebra fish.
Scientific Background: EP300 (E1A-associated protein p300, also known as p300 or KAT3B) is a large (265 kDa) multifunctional transcriptional coactivator and histone acetyltransferase (HAT) that plays a central role in regulating cell growth, proliferation, differentiation, and apoptosis. It acts as a molecular scaffold to connect transcription factors to the basal transcription machinery. It features a bromodomain, a RING-PHD (CH2) domain, and a catalytic HAT domain. It is closely related to the CREB-binding protein (CBP) and and like CPB can stimulate transcription through activation of CREB. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. It regulates transcription via chromatin remodeling through acetylation all four core histones in nucleosomes. It functions as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and participates in the stimulation of hypoxia-induced genes such as VEGF. It can be methylated at R580 and R604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates the apoptotic response. It is also methylated at R2142 by CARM1, which impairs interaction with NCoA2. It is specifically inhibited by the adenovirus oncoprotein E1A. It binds to and may be involved in the transforming capacity of the adenovirus E1A protein. Rubinstein-Taybi Syndrome 2 is caused by heterozygous variants in EP300. It is involved in various cancers, including epithelial cancer and diffuse large B-cell lymphoma (DLBCL), where it is often mutated. This description may include information annotated by UniProt and/or Google AI.