Product Name:
DPYD-pS766+pT768
Product Number:
ab-pn747
Target Full Name: Dihydropyrimidine dehydrogenase [NADP()]
Target Alias: DHP; DHPDHase; Dihydropyrimidine dehydrogenase; Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase; DPD; EC 1.3.1.2; MGC70799; OTTHUMP00000058954
Product Type Specific: DPYD phosphosite-specific antibody
Antibody Code: PN747
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S766+T768
Protein UniProt: Q12882
Protein SigNET: DPYD
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human DPYD
Antibody Immunogen Sequence: CGGV(pS)G(pT)AIR
Antibody Immunogen Description: Corresponds to amino acid residues G763 to R771. The effects of S766 and T768 phosphorylation are unclear. These phosphorylation sites have only been detected in a single mass spectrometry study. However, this human phosphosite is highly conserved in vertebrates and also found in sea urchin.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse | Chicken | Zebra fish | Fruit fly | Honey bee | Nematode worm | Sea urchin.
Scientific Background: DPYD (Dihydropyrimidine dehydrogenase [NADP(+)]) is an oxidoreductase that belongs to the dihydropyrimidine dehydrogenase family. It is a homodimer, with each subunit (~111 kDa, 1025 amino acids) containing FMN, FAD, and [4Fe-4S] clusters. It is Involved in pyrimidine base degradation, and catalyzes the reduction of uracil and thymine into 5,6-dihydrotymine. An example of a reversible reaction catalyzed by DPYD is: 5,6-dihydrouracil + NADP+ => uracil + NADPH + H+. DPD is responsible for over 80% of the metabolism of 5-fluorouracil (5-FU), a common chemotherapy agent, and its prodrug, capecitabine. Patients with genetic mutations in DPYD (e.g., c.1905+1G>A), c.1679T>G, c.2846A>T, and HapB3) may have reduced DPD activity, causing 5-FU to accumulate, which can lead to severe, potentially fatal toxicities (e.g., severe diarrhea, neutropenia, neurotoxicity). Such inactivating mutations can cause a complete or partial deficiency, with partial deficiency affecting 2-8% of the population. DPYD is cytoplasmic and is expressed in most tissues with highest levels found in liver and peripheral blood mononuclear cells. This description may include information annotated by UniProt and/or Google AI.